【作者】 陶敏慧；

【导师】 杨绍斌；

【作者基本信息】 辽宁工程技术大学 ， 矿物加工工程， 2009， 硕士

【摘要】 传统生物浸出难浸金矿工艺中细菌与矿物直接接触,矿浆中逐渐积累的有毒有害离子、搅拌产生的巨大的剪切力及矿物对O₂和CO₂传递的阻碍等作用抑制细菌生长繁殖和生物活性,造成其对矿物和Fe2+氧化速率降低,延长生物浸出周期,限制生物浸出技术在工业上的推广应用。为了改善浸矿周期长的问题,从上述现象入手,基于间接浸出机理,本实验采用有效分离间接生物浸出（Indirect Bioleaching with Effects Separation,IBES）工艺,将细菌生长过程和细菌氧化Fe2+产物Fe3+化学浸出难浸金矿过程分开,分别控制两段反应条件,使各自在最佳条件下进行,提高各段反应速率,进而缩短整个反应周期。实验中的生物反应阶段采用固定化细胞技术提高浸矿菌活性,固定化载体为聚氨酯泡沫,用于固定化的浸矿菌为嗜中温氧化亚铁硫杆菌（ Acidithiobacillus ferrooxidants, At.f）和中度嗜热西伯利亚硫杆菌（Sulfobacillus sibiricu,S,s）,用于浸出的目标矿物为难浸高砷金精矿。实验结果表明,利用聚氨酯泡沫固定化中度嗜热西伯利亚硫杆菌大幅度提高该菌对Fe2+的氧化速率,在温度为50℃,通气量为4 L/min的条件下,固定化中度嗜热西伯利亚硫杆菌在5h内将浓度为10g/L的Fe2+转化95%,约是相同条件下,接种量为10%的该游离菌摇瓶实验反应时间（36h）的七分之一。酸性硫酸铁溶液浸出高砷金精矿的速率优于细菌直接浸矿体系,70℃,10g/L的酸性硫酸铁溶液连续浸出高砷金精矿,4天内As的浸出率达到71%.实验证明采用有效分离间接生物浸出工艺浸出高砷金精矿更为有利。更多还原

【Abstract】 In the traditional bioleaching progress of refractory gold ore, bacteria lives with pulp density directly, the steadily accumulated poison and harmful ion、the huge shearing force produced by stirring and the high density blocks the transformation of O₂ and CO₂, all these affect bacteria growth and activity, leading to low oxidation rate of Fe²⁺, longing the leaching time, which limit the apply of the bioleaching technology.In order to increase the leaching rate, base on the indirect mechanism, this thesis adopts Indirect Bioleaching with Effects Separation process, imparts the two stages of bioleaching, so that both stages can react in their own condition which can rise the rate of two stages separately, shorter the whole reaction time. In the experiment, we use cell immobilization technique to enhance the bacteria activity, the carrier is polyurethane foam, bacteria using in the immobilization are Acidithiobacillus ferrooxidants and Sulfobacillus sibiricu,the target mineral is rich-arsenic gold concentration. The result is, the rate of S.s oxidize Fe²⁺ is raised by immobilization, under the air rate of 4L/min, 10g/L Fe²⁺ converse 95% takes 5h by immobilization S.s, which is one-seventh of the 10% free S.s （36h）. The system of acid ferric sulfate solution leaching rich-arsenic gold concentration is better than bioleaching system, at 70℃, the leaching rate of rich-arsenic gold concentration achieve 71% takes 4 days by 10g/L Fe3+ solution. The result show that IBES progress leaching rich-arsenic gold concentration is more effective.更多还原