【作者】 王一郎；

【导师】 田坚；

【作者基本信息】 长春理工大学 ， 生物医学工程， 2010， 硕士

【摘要】 为了在高灵敏度和特异性下快速检测出微量水平的病毒,人们发明了银表面增强拉曼光谱(surface enhanced Raman scattering, SERS)技术。这种技术能分辨在生物体中的病毒、病毒链和被剔除部分基因的病毒在拉曼光谱上的不同。无需对病毒进行复杂的操作,这种技术就可以快速检测和描绘出由病毒引起的拉曼光谱信息,然而这种技术的关键之处在于能制备出实用并且具有较强SERS效应的基底材料。本文以平均直径为200nm的聚苯乙烯微珠作为SERS的基底材料,用化学和物理方法使其表面均匀沉积一定厚度的银,然后检测嘧啶和嘌呤碱基的拉曼信号。更多还原

【Abstract】 A spectroscopic assay based on surface enhanced Raman scattering (SERS) using silver nanorod array substrates has been developed that allows for rapid detection of trace levels of viruses with a high degree of sensitivity and specificity. This novel SERS assay can detect spectral differences between viruses, viral strains, and viruses with gene deletions in biological media. The method provides rapid diagnostics for detection and characterization of viruses generating reproducible spectra without viral manipulation, but the key of this spectroscopic assay is how to make the practical,feasible and effective substrate material for SERS.This paper chooses the tiny balls of polystyrene as the substrate material of SERS. The size of the tiny balls of polystyrene ranged from 150 to 250 nm (mean 200nm) in diameter, through chemical and physical methods it deposits a layer of Ag on the tiny balls, and then use it to to detect and measure Raman spectrum of pyrimidine and purine bases.更多还原