【作者】 汪飞；

【导师】 孙汉文；

【作者基本信息】 河北大学 ， 分析化学， 2009， 硕士

【摘要】 喹诺酮类药物是用于泌尿、呼吸道、胃肠道。骨关节及皮肤等多种组织感染的抗微生物药物,近年来得到广泛的应用,但是滥用喹诺酮类药物,不仅增加了生物的抗药性,还给环境造成了一定的影响,危害到人们的健康,因此,建立一种简单、灵敏、快速、准确的测定喹诺酮类药物的方法非常必要,本文根据喹诺酮类药物的化学发光行为,建立了流动注射化学发光测定喹诺酮类药物的分析方法,并对机理进行了探讨,对以后的工作奠定了一定的基础。全文分为：第一章：论述了化学发光分析法的历史及原理,并介绍了对喹诺酮类药物的研究进展。第二章：提出了测定恩诺沙星的化学发光新方法,采用恩诺沙星-KMnO4-Na2S2O3-H2SO4化学发光体系,成功的测定了恩诺沙星注射液和片剂中恩诺沙星的含量,回收率在97.3%-105.1%和90.1%～103.6%之间,结果令人满意。第三章：研究了新的化学发光体系-Ag(Ⅲ)-H2SO4,并用于恩诺沙星的测定,试验中不仅测定了片剂及注射液中恩诺沙星的含量而且对此体系的机理进行了探讨,建立了快速灵敏的测定恩诺沙星的化学发光新方法。第四章：利用诺氟沙星对Ag(Ⅲ)-H2SO4体系的增敏作用,我们建立了测定诺氟沙星的化学发光方法,该方法检出限为3.097×10-9g/mL,对2.0×10-7g/mL诺氟沙星进行11次平行测定,相对标准偏差为1.7%。回收率在95.0%-104.0%.利用该方法测定了血清,尿液和胶囊中诺氟沙星,结果令人满意。更多还原

【Abstract】 Quinlones are the new generation broad-spectrum antibities used in the treatment of infections of urinary tract, respiratory tract, gastroenteritis, skin and skin structures infections, they are developed quickly and widely used for clinic in recent years, but this result in not only the environment to be contaminated, but also the resistence to be increased, even endenger for the human life. Thus, it is necessary to studied and founded the quick and sensitive analysis methods of quinlones. This thesis studies the chemiluminescence (CL) behavior of quinolones, and used the flow injection CL methods for the determination of some quenolones. The CL mechanism was discussed. This dissertation includes four chapters as follows:Chapter 1:The history and fundamental principle of CL method are reviewed and introduced the CL methods for quinolones in details.Chapter 2:A chemiluminescence system-enrofloxacin - KMnO4- Na2S2O3 - H2SO4, was developed for the determination of enrofloxacin. The method has been applied to the determination of enrofloxacin tables and injections with satisfied results. and the recovery is between 97.3%-105.1% and 90.1%～103.6%.Chapter 3:The chemiluminescence reaction of Ag (Ⅲ)-H2SO4 system was studied. Based on it, a new method for the determination of enrofloxacin was developed. In this enperiment, we determined the enrofloxacin tables and injections and discussed the the mechinism.Chapter 4:Norfloxacin can enhance the CL signals of Ag (Ⅲ)-H2SO4 system, a novel rapid flow injection method with chemiluminescence detection was established for the determination of norfloxacin. The detection limit was 3.097×10-9g/mL, and the relative standard deviation (n=11) is 1.7%at 2.0×10-7g/mL. The recovery was 95.0%～104.0%, This method was used for the determination of norfloxacin in the serum, urine and capsule with satisfactory results.更多还原