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亚低温对番茄hta9hta11双突变体光合碳同化相关指标的影响

Effect of mild hypothermia on photosynthetic carbon assimilation related indexes of hta9hta11 double mutant of Lycopersicon esculentum

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【作者】 卢盼玲郭世荣杨学东冯岩季维维张辉田守波朱为民

【Author】 LU Panling;GUO Shirong;YANG Xuedong;FENG Yan;JI Weiwei;ZHANG Hui;TIAN Shoubo;ZHU Weimin;College of Horticulture,Nanjing Agricultural University;Shanghai Key Lab of Protected Horticultural Technology,Horticultural Research Institute,Shanghai Academy of Agricultural Science;Shanghai Xinghui Seedling Co.,Ltd.;

【通讯作者】 朱为民;

【机构】 南京农业大学园艺学院上海市农业科学院设施园艺研究所上海市设施园艺技术重点实验室上海星辉种苗有限公司

【摘要】 <正>表观遗传机制可调控植物对逆境胁迫的应答,组蛋白变体H2A.Z为重要的表观修饰因子,参与多种非生物胁迫[1-3],并且,其基因家族成员较多[4-5]。hta9hta11双突变体植株具有多效性表型,且H2A.Z基因家族其他成员的表达不能补偿HTA9和HTA11蛋白功能缺失[6]。番茄(Lycopersicon esculentum Mill.)为重要的设施蔬菜和常用的模式植物,对低温敏感[7]。亚低温条件下番茄hta9hta11双突变体

【Abstract】 Taking day temperature of 25 ℃ and night temperature of 20 ℃ as the control group, variations of photosynthetic carbon assimilation related indexes of wild type and hta9 hta11 double mutant of Lycopersicon esculentum Mill. in treatment group(day temperature of 15 ℃ and night temperature of 10 ℃) were compared. The results show that chlorophyll content in hta9 hta11 double mutant in treatment group is slightly lower than that of wild type; its net photosynthetic rate, contents of sucrose and starch, activities of fructose-1,6-bisphosphate aldolase and fructose-1,6-bisphosphatase, and relative expression levels of TK, GAPDH, SBPase, PRK, RbcL, RCA, FBPase and FBA genes are significantly lower than those of wild type; while its ribulose-1,5-bisphosphate carboxylase/oxygenase activity and relative expression level of RbcS gene are significantly higher than those of wild type. It is suggested that photosynthetic carbon assimilation ability of hta9 hta11 double mutant of L. esculentum is lower than that of wild type under mild hypothermia condition, and histone variant H2 A.Z may play an important role in regulating photosynthetic carbon assimilation pathway of L. esculentum.

【基金】 国家重点研发计划项目(2016YFD0101703; 2017YFD0101902);上海市农委资助项目[沪农科攻字(2015)第6-1-7号;沪农科种字(2017)第3-1-2号;沪农青字(2018)第1-15号]
  • 【文献出处】 植物资源与环境学报 ,Journal of Plant Resources and Environment , 编辑部邮箱 ,2019年02期
  • 【分类号】S641.2
  • 【下载频次】143
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