【摘要】 以4年生钩藤为实验材料,研究了不同浓度的乙烯对其生理以及主要药用成分（钩藤碱和异钩藤碱）积累的影响。结果表明:外施乙烯能抑制钩藤净光合速率、诱导气孔关闭、降低蒸腾速率、提高胞间CO2浓度、显著提高叶中脯氨酸含量,但对叶中可溶性糖、丙二醛及叶绿素含量影响较小;同时,乙烯对钩藤生理的影响与处理时间有关,双因素方差F值显示,导致叶中脯氨酸、可溶性糖以及丙二醛积累的主导因素均为处理时间。乙烯对主要药用成分积累的调控表现出二重性, 50和500 mg·L^-1处理促进叶片和带钩茎枝中主要药用成分的积累,而浓度为2 000 mg·L^-1时,则表现为抑制积累;调控还呈现器官差异性, 1 000 mg·L^-1处理抑制叶片主要药用成分的积累,但促进其在带钩茎枝中的积累;导致叶中主要药用成分变化的主导因素是浓度,而在带钩茎枝中主导因素为处理时间。此外,乙烯的调控作用,能让同一部位的钩藤碱和异钩藤碱含量发生同步变化。更多还原

【Abstract】 To study the effect of ethylene with different concentrations on physiology and accumulation of main medicinal components（rhynchophylline and isorhynchophylline）, four-year-old Uncaria rhynchophylla was carried out as the experimental materials. The results showed that ethylene inhibited net photosynthetic rate, induced stomatal closure, decreased transpiration rate, increased intercellular CO2 concentration, especially it increased the proline content in Uncaria rhynchophylla leaves significantly, but had little effect on soluble sugar, malondialdehyde and chlorophyll content. Moreover, the physiological effect was related to treatment time. F value of two-factor variance analysis indicated treatment time was the main factor which caused the accumulation of proline, soluble sugar and malondialdehyde in leaves. The regulation of ethylene on the accumulation of main medicinal components showed duality. The accumulation of main medicinal ingredients in leaves and branches with hooks was promoted by 50 and 500 mg·L^-1 treatments, but inhibited by 2 000 mg·L^-1. This regulation also showed organ differences. 1 000 mg·L^-1 treatment inhibited the accumulation of main medicinal components in leaves, but promoted their accumulation in branches with hooks. The concentration was the dominant factor leading to the change of the main medicinal components in leaves, while the treatment time was the dominant factor in the branches with hooks. In addition, the regulation of ethylene can change the contents of rhynchophylline and isorhynchophylline in the same part synchronously.更多还原