【摘要】 热激转录因子(heat shock transcription factors, Hsfs)在植物遭受非生物胁迫时调控热激蛋白(heat shock protein, Hsp)和其他热激诱导的转录本的表达,参与响应热胁迫进程。本文以香石竹‘想象’作为实验材料,对基因DcHsfB1开放阅读框进行克隆。氨基酸序列的组成成分及理化性质分析结果表明, DcHsfB1含有一个804 bp的开放阅读框,编码267个氨基酸,分子量为30.78 kDa,理论等电点为5.84。DcHsfB1蛋白的二级及三级结构预测表明,α-螺旋占36.36%,延伸链占13.82%,β-折叠链占4.0%,无规则卷曲占45.82%,无规则卷曲构成了蛋白质的主要骨架。系统进化树分析结果显示DcHsfB1与AtHsfB1同源性最高。实时定量PCR分析DcHsfB1在各种非生物胁迫下的相对表达量。结果表明,在42°C胁迫2 h、ABA处理3 h和干旱胁迫3 h的Dc Hsf B1相对表达量显著上调;在甘露醇、NaCl和4°C胁迫下DcHsfB1的相对表达量也有不同程度的上升。更多还原

【Abstract】 Heat shock transcription factors(Hsfs) regulate the expression of heat shock proteins(Hsps) and other heat shock-induced transcripts when plants are subjected to abiotic stress, and participate in the response to heat stress. In this paper, carnation ‘Fancy’ was used as experimental material to clone the open reading frame of DcHsfB1. The composition and physicochemical properties analysis of the amino acid sequence showed that DcHsfB1 contained a 804 bp open reading frame encoding 267 amino acids with a molecular weight of 30.78 kDa and a theoretical isoelectric point of 5.84. The secondary structure and tertiary structure prediction of the protein showed that, α-helix accounted for 36.36%, extended chain accounted for 13.82%, β-folded chain accounted for 4.0%, and irregular curl accounted for 45.82%. Irregular curl constituted the main skeleton of the protein. Phylogenetic tree analysis showed that DcHsfB1 had the highest homology with AtHsfB1. The relative expressions of DcHsfB1 under various abiotic stresses were analyzed by quantitative real-time PCR. The results showed that the relative expression of the gene were significantly up-regulated under 42°C for 2 h, ABA treatment for 3 h and drought stress for 3 h. The relative expression of DcHsfB1 under mannitol and NaCl and 4°C also increased in different degrees.更多还原