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抗玉米蚜玉米内生菌的筛选

Screening of Corn Endophyte Strains against Rhopalosiphum maidis

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【作者】 孙艺昕门兴元李超于毅吕素洪孙廷林叶保华李丽莉

【Author】 SUN Yixin;MEN Xingyuan;LI Chao;YU Yi;Lü Suhong;SUN Tinglin;YE Baohua;LI Lili;Key Laboratory for Plant Virology of Shandong, Shandong Academy of Agricultural Sciences;College of Plant Protection, Shandong Agricultural University;

【通讯作者】 叶保华;李丽莉;

【机构】 山东省农业科学院植物保护研究所/山东省植物病毒学重点实验室山东农业大学植物保护学院

【摘要】 为探索防治玉米蚜的新方法,从山东省玉米主产区玉米植株上分离获得61株内生菌菌株,室内测定了对玉米蚜的防治效果,筛选出高效菌株,并测定了菌株对玉米生长的影响及在玉米植株内的定殖率。结果显示,白僵菌属菌株YC1和链格孢属菌株GX5对玉米蚜的防治效果最好,均为74.80%,无虫株率分别为32.22%和55.64%;接入菌株YC1和GX5后玉米各组织的生物量均大于对照,菌株YC1处理组的根、茎、叶干重分别增长了9.64%、15.71%和20.51%,菌株GX5处理组的根、茎、叶干重分别增长了3.88%、8.16%和9.14%;菌株GX5在玉米根、茎、叶中的定殖率可以达到44.23%以上,菌株YC1在玉米茎中的定殖率可以达到63.44%,有较大的应用价值。

【Abstract】 To explore new strategies for sustainable control of corn aphid Rhopalosiphum maidis(Fitch) population,61 endophyte strains were collected from the major corn producing areas in Shandong province, and their control efficacies against R. maidis were determined under laboratory conditions to screen the highly effective strains.Meanwhile, the effects and rates of colonization of the strains in different tissues of corn were detected. The results showed that strains YC1 and GX5 had the best control efficacy of 74.80%, with rates of no-damage plants of32.22% and 55.64%, respectively. The biomass of corn tissues inoculated with stains YC1 and GX5 were higher than the control, dry weight of the root, stem and leaf of the YC1 treatment group increased by 9.64%, 15.71% and20.51%, respectively, and that of the GX5 treatment group increased by 3.88%, 8.16% and 9.14%, respectively. The colonization rates of strain GX5 in roots, stems and leaves were more than 44.23%, and the colonization rate of YC1 in stems was more than 63.44%. The results show that the strains GX5 and YC1 have great application potential.

【基金】 国家重点研发计划(2018YFD0200603);山东省农科院创新工程(CXGC2019G01,CXGC2016A09)
  • 【文献出处】 中国生物防治学报 ,Chinese Journal of Biological Control , 编辑部邮箱 ,2019年04期
  • 【分类号】S435.132;S476
  • 【网络出版时间】2019-06-28 16:03
  • 【被引频次】5
  • 【下载频次】355
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