【摘要】 目的对福建地区分离的猪源大场杆菌进行耐药性测定以及对ESBLs流行性进行分析,以期为临床合理用药科学提供依据。方法采用琼脂稀释法测定373株菌大肠杆菌对12种抗菌药物的敏感性,并用PCR方法检测ESBLs耐药基因的携带情况以及分析CTX-M阳性菌的种系发育关系。结果分离株对甲氧苄啶/磺胺甲噁唑、萘啶酸、氟苯尼考及氨苄西林耐药率较高,分别为93.8%、82.8%、73.7%和70.0%;对恩诺沙星、四环素、庆大霉素、多西环素、环丙沙星、卡那霉素、头孢噻呋和头孢噻肟的耐药率依次为54.4%、53.1%、52.0%、46.6%、44.0%、41.0%、35.9%和30.8%。在373株大肠杆菌中,共有122株菌携带有ESBLs耐药基因,检出率为32.7%,其中CTX-M、TEM及OXA的检出率分别是18.0%(67)、12.1%(45)和7.5%(28),其中CTX-M的阳性菌中以CTX-M-14最为流行,其次是CTX-M-65和CTX-M-55;其种系发育显示分离株主要分布在A群。结论福建地区猪大肠杆菌对抗菌药表现出较强的耐药性,ESBLs在猪源大肠杆菌中广泛流行,CTX-M是其主要流行的基因型,并且多数菌以共生型大肠杆菌为主。更多还原

【Abstract】 The aim of this research was to study the prevalence of antimicrobial resistance and distribution of extended-spectrum-β-lactamaeses(ESBLs)genes of the Escherichia coli isolated from swine,and provided guidance for the rational use of antibiotics.A total of 373 isolates were tested for antimicrobial susceptibility to 12 antimicrobial agents by agar dilution method,screened for ESBLs genes and analysed phylogenetic group among the CTX-M positive isolates by PCR.Results showed strains were found high resistance rates to trimethoprim/sulfamethoxazole,nalidixic acid,florfenicol and ampicillin,with resistance rates of 93.8%,82.8%,73.7%and 70.0%,respectively.Strains had showed resistance to enrofloxacin,tetracycline,gentamicin,doxycycline,ciprofloxacin,kanamycin,ceftiofur and cefotaxime,and the resistance rates were 54.4%,53.1%,52.0%,46.6%,44.0%,41.0%,35.9% and 30.8% as follows.There were 32.7%(122/373)of the isolates carried ESBLs genes,including CTX-M(18.0%,67),TEM(12.1%,45)and OXA(7.5%,28)genes,and the CTX-M-14 was the most predominant subtype during the encoding CTX-M isolates,then of CTX-M-65 and CTX-M-55.Phylogeny showed that the strains were mainly distributed in the A group.This research revealed that high rates of resistance to antimicrobial agents,and ESBLs was widely prevalent in Escherichia coli isolated from Fujian Province.The CTX-M was the most predominant gene among those strains,and the majority of them belonged to symbiotic Escherichia coli.更多还原