【摘要】 目的:运用生物信息学方法分析电离辐照损伤小鼠的睾丸组织,提取出小鼠辐照组与正常组睾丸组织比较后的差异表达蛋白质,分析其功能和生物学过程。方法:建立小鼠电离辐照损伤模型。运用比较蛋白质组学方法、i TRAQ联合LC-MS/MS检测技术筛选出差异表达蛋白质,进一步利用David、Uniport数据库对差异蛋白进行GO、KEGG富集分析。结果:通过比较小鼠辐照组与正常组睾丸组织共鉴定出蛋白质1633个,包含差异蛋白128个,其中28个表达上调,100个表达下调。GO分析显示差异蛋白主要涉及氧化还原过程、精子发生、ATP代谢等生物学过程。KEGG分析显示主要分布在帕金森氏病、氧化磷酸化、代谢途径等21条信号通路中。结论:利用生物信息学方法对电离辐照损伤小鼠睾丸组织的蛋白质信息进行挖掘,为进一步研究电离辐照对睾丸组织的辐射损伤机制及寻找生物标志物提供参考。更多还原

【Abstract】 Objective:To investigate the differentially expressed proteins between mouse testicular tissue with ionizing radiation injury and normal testicular tissue using bioinformatics methods and the functions and biological processes of these proteins. Methods:A mouse model of ionizing radiation injury was established. Comparative proteomic methods,isobaric tags for relative and absolute quantitation,and liquid chromatography-tandem mass spectrometry were used to screen out differentially expressed proteins,and then David and Uniport databases were used for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genome(KEGG)enrichment analyses of differentially expressed proteins. Results:A total of 1633 proteins were identified in mouse testicular tissue with ionizing radiation injury and normal testicular tissue,with 128 differentially expressed proteins in total,among which 28 were upregulated and100 were downregulated. GO analysis showed that the differentially expressed proteins were mainly involved in biological processes such as oxidation-reduction process,spermatogenesis,and ATP metabolism. KEGG analysis showed that these proteins were mainly distributed in 21 signaling pathways including Parkinson ’s disease,oxidative phosphorylation,and metabolic pathways,etc.Conclusion:The bioinformatics method is used for data mining of proteins in mouse testicular tissue with ionizing radiation injury and normal testicular tissue,which provides a reference for further research on the mechanism of ionizing radiation in inducing testicular tissue injury and new biomarkers.更多还原