节点文献
河川沙塘鳢GH基因及侧翼的克隆与生物信息学分析
Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila
【摘要】 利用长片段PCR、基因组步移法及T-A克隆技术,成功克隆出河川沙塘鳢(Odontobutis potamophila)GH基因全长序列,提交至GenBank获得登录号MH717101。河川沙塘鳢GH基因全序列长5 120 bp,5′端和3′端侧翼序列长度分别为920、682 bp,经预测,上游区域含有MF3、GAGA factor、HNF-3alpha、C/EBP、R2、GATA-1等多种转录调控元件;转录单元长3 518 bp,包含4个内含子和5个外显子,4个内含子大小分别为75、333、2 070和121 bp,5个外显子长度分别为150、131、117、138和373 bp,第3内含子长达2 070 bp,其长度是翘嘴鲌(Culter alburnus)第3内含子的4倍以上;开放阅读框区(open reading frame,ORF)为594 bp,编码由197个氨基酸残基组成的蛋白质多肽。河川沙塘鳢与鲈形目、鲉形目、鲤形目、鲇形目等13种鱼类的氨基酸序列同源性比较表明,其与彼氏冰鰕虎鱼(Leucopsarion petersii)同源性最高,为83.8%,与草鱼(Ctenopharyngodon idella)同源性最低,为49.1%,在180~197氨基酸序列处,河川沙塘鳢表现出高度不保守性,与所列鱼类氨基酸排列均完全不一致。该研究结果为进一步研究河川沙塘鳢GH基因的表达、功能及其转录调控特征奠定了分子基础。
【Abstract】 The full-length sequence of the Odontobutis potamophila GH gene was successfully cloned by using long segment PCR, genome walking method and T-A cloning technology, which was submitted to GenBank to obtain the login number MH717101. The full-length of the GH gene of Odontobutis potamophila is 5 120 bp, the length of the 5′-end and 3′-end lateral sequence is 920 bp and 682 bp. It is predicted that the upstream region contains MF3, GAGA factor, hnf-3 alpha, C/EBP, R2, GATA-1 and other transcriptional regulatory elements, respectively. The transcription unit is 3 518 bp long and contains 4 introns and 5 exons. 4 introns are 75, 333, 2 070 and 121 bp; 5 exons are 150, 131, 117, 138 and 373 bp, respectively. The third intron is 2 070 bp, the length was more than 4 times of the culter third intron. The open reading frame area(ORF) is 594 bp, encoding a protein polypeptide composed of 197 amino acid residues. Comparison of amino acid sequence homology between Odontobutis potamophila and Perciformes, Scorpaeniformes, Cypriniformes, Siluriformes with 13 fishes showed that the homology was the highest to Leucopsarion petersii, at 83.8%, and the homology to Ctenopharyngodon idella was the lowest, at 49.1%, the Odontobutis potamophila to show highly nonconservative at 180-197 amino acid sequence and the amino acid arrangement was completely different with the listed fish. The results of this study laid a molecular foundation for further study on expression, function and transcriptional regulation of GH gene in Odontobutis potamophila.
【Key words】 Odontobutis potamophila; growth hormone; clone; sequence analysis; bioinformatics;
- 【文献出处】 浙江农业学报 ,Acta Agriculturae Zhejiangensis , 编辑部邮箱 ,2019年09期
- 【分类号】S917.4
- 【网络出版时间】2019-08-23 16:17
- 【被引频次】2
- 【下载频次】114