【摘要】 目的了解从地震受伤患者分离到的耐甲氧西林金黄色葡萄球菌(MRSA)基因特征,并与同期非灾区患者分离的MRSA进行比较。方法用葡萄球菌盒式染色体(SCCmec)多重PCR分型技术进行基因分型,并检测其杀白细胞毒素基因(PVL),采用Diversilab进行同源性比较,并进行药敏分析。结果地震受伤患者与非灾区患者分离到的MRSA均表现为SCCmecⅢ型,PVL阴性;同源性分析显示,分离到的MRSA与社区获得性MRSA代表株中MLST分型ST8型同源性相对较好。结论医院获得性MRSA与社区获得性MRSA可能存在交叉传播,在灾后急救现场及当地医院要加强无菌观念,高度警惕医源性交叉感染的传播。更多还原

【Abstract】 OBJECTIVE To investigate the genetic feature of meticillin-resistant Staphylococcus aures isolated from earthquake victims and compare them with those of common patients.METHODS The MRSA isolates underwent SCCmec(Staphylococcal Cassette Chromosome mec) multi-PCR and PVL gene detection.Diversilab method was used for homology comparison and antibiotic resistance was also analyzed.RESULTS All the MRSA isolates were confirmed to be type Ⅲ SCCmec with PVL gene negative.Homology analysis showed relatively consistent with community-acquired MRSA confirmed to be ST8 by MLST typing.CONCLUSIONS There may be cross-spread of MRSA between hospital and community.We should pay more attation even in emergent time.更多还原