【摘要】 根腐病是制约石柱县黄连产业发展的主要病害之一。为了明确根腐病病根中真菌组成及致病菌情况,为该病的防治提供数据,该研究在不同季节采集石柱4个黄连主产区一至五年生的黄连根腐病病根进行真菌分离,对分离到的菌株进行分子鉴定、形态鉴定和种属统计。结果共计分离到真菌437株,分别属于5亚门,11纲,16目,22科,28属。黄连根腐病病根部位可培养真菌数量、种类随生长年限、地点、季节而变化,但未显示出明显变化规律。Ilyonectria sp.,Pythium sp.,Phoma sp,Trichoderma sp.是优势属。结合前期初步离体回接结果推断,Pythium sp.,Ilyonectria sp.,Phoma sp.,Fusarium sp.中都可能包含病原菌。Trichoderma sp.中可能筛选出根腐病菌的拮抗菌。更多还原

【Abstract】 Root rot disease restricts the Coptis chinensis industry in Shizhu of Sichuan province. To disclose fungi composition and pathogen in rot root,so as to prevent and treat the root rot disease,the C. chinensis rot root of 5 years from 4 areas in Shizhu were collected in 3 seasons respectively. The fungi were isolated and molecularly and morphological identified,followed with population statistics. 437 fungi were isolated,belonging to 5 subphylum,11 classes,16 orders,22 families and 28 genus respectively. There are great difference among the fungi compositions of different area,year and sampling season,while there was no obvious variation rule. Ilyonectria sp.,Pythium sp.,Phoma sp,Trichoderma sp.are dominant genus,while Pythium sp.,Ilyonectria sp.,Phoma sp.,Fusarium sp. may contain root rot pathogen. Antagonistic bacteria may be screened from the strains of Trichoderma sp. isolated.更多还原