【摘要】 目的检测不同干预措施后急性过敏性气道炎症小鼠的气道中三叶因子1(TFF1)、三叶因子3(TFF3)的表达水平,并初步探讨重组TFF3对气道炎症反应及黏液分泌水平的影响。方法 40只BALB/c小鼠随机分为生理盐水对照组(A组)、急性过敏性气道炎症组(B组)、布地奈德干预组(C组)、TFF3重组体干预组(D组)、TFF3+布地奈德干预组(E组),每组8只。卵清蛋白致敏及激发构建小鼠急性过敏性气道炎症期模型,苏木精–伊红染色光镜下观察肺组织炎症情况,阿辛蓝染色分析气道黏液分泌情况,免疫组织化学分析气道TFF1、TFF3表达情况。结果气道炎症评分及黏液分泌情况:B组显著大于A组(P<0.01),C组小于B组(P<0.05),D组与B组相比无明显差异(P>0.05),C组与E组相比无明显差异(P>0.05)。TFFs的表达:TFF1、TFF3在A、B、C组支气管及细支气管上皮细胞、杯状细胞及黏膜下腺细胞中均有表达;其中B组的TFF1及TFF3表达均显著高于A组(P<0.01),C组的TFF1及TFF3表达低于B组(P<0.05)。气道上皮TFF1水平与气道炎性评分、黏蛋白表达水平均呈显著正相关(r=0.876、0.807,均P=0.000),TFF3水平与炎性评分、黏蛋白表达水平均呈显著正相关(r=0.654,P=0.006;r=0.666,P=0.005)。结论小鼠急性过敏性气道炎症期气道TFF1、TFF3表达增高。经鼻滴入外源性TFF3对气道炎症及黏液分泌均无明显影响,吸入糖皮质激素可一定程度抑制TFF1和TFF3的表达,同时抑制气道炎症及黏液分泌。更多还原

【Abstract】 Objectives To detect expressions of trefoil factor 1(TFF1) and TFF3 in the mice with acute allergic airway disease(AAD) after different interventions, and explore primitively the effect of recombinant TFF3 on airway inflammation and mucous secretion. Methods Forty BALB/c mice were randomly divided into 5 groups, each group with 8 mice, ie. a normal saline control group(group A), an AAD group(group B), a budesonide intervention group(group C), a recombinant TFF3 intervention group(group D), and a budesonide+recombinant TFF3 intervention group(group D). The BALB/c mice were sensitized and challenged with ovalbumin to induce AAD. Lung tissue sections were stained with hematoxylin-eosin staining for assessment of airway inflammation, and immunohistochemistry was used for detecting TFF1/TFF3 expression in the airway. Alcian blue stain was applied to determine mucous secretion. Results Airway inflammation score and airway mucous secretion: Group B was significantly more than group A(P<0.01); Group C was less than group B(P<0.05), and there was no significant difference between group D and group B(P>0.05); There was no significant difference between group C and group E(P>0.05). Expression of TFFs: TFF1 and TFF3 were expressed in epithelial cells, goblet cells and submucosal gland cells of bronchi and bronchioles in all groups; The expressions of TFF1 and TFF3 in group B were significantly higher than those in group A(P<0.01), while the expressions of TFF1 and TFF3 in group C were lower than those in group B(P<0.05). TFF1 expression in airway epithelium was positively correlated with inflammatory score(r=0.876, P=0.000) and mucin expression(r=0.807, P=0.000). TFF3 level was positively correlated with inflammatory score(r=0.654, P=0.006) and mucin expression(r=0.666, P=0.005). Conclusions Ovalbumininduced acute allergic airway inflammation significantly increases TFF1/TFF3 expression. Intranasal TFF3 treatment may not influence airway inflammation and mucus secretion. Inhaled corticosteroids to some extent inhibit expressions of TFF1 and TFF3, simultaneously suppress airway inflammation and mucus secretion in the mouse model of acute AAD.更多还原