【摘要】 研究了厌氧反硝化产甲烷体系中,典型含氮杂环化合物喹啉、吲哚作为共基质碳源,厌氧生物对二者的降解特性,及群落分析.结果表明:在共基质条件下,喹啉的存在对吲哚的生物降解有抑制作用,且抑制随喹啉浓度的升高而升高;吲哚的存在对喹啉的生物降解有促进作用,但吲哚浓度过高(150mg/L)抑制了喹啉的降解;喹啉、吲哚共基质时,二者的降解都遵循零级反应动力学;通过GC-MS分析,喹啉的主要中间代谢产物分别为2(1H)喹诺酮与8-羟基-2(1H)喹诺酮;吲哚的主要代谢产物为2-吲哚酮与靛红;通过高通量测序对共基质体系的微生物群落进行分析,发现厌氧功能菌群得到富集,细菌菌门以变形菌门Proteobacteria为主,菌纲以Gammaproteobacteria和Betaproteobacteria为主,菌属以Acinetobacter,Candidimonas,Azospira,和Desulfomicrobium为主.更多还原

【Abstract】 Bath experimrnts were conducted to study the degradation characteristics and microbial community of quinoline and indoles co-substrate under anaerobic denitrification and methanogenesis conditions, which were known as typical N-heterocyclic compounds. The results showed that the presence of quinolone could inhibit the degradation of indole, and the inhibition effect was enhanced with the increase of quinoline concentration; the presence of indole could promote the degradation of quinoline, but the high concentration of indole(150 mg/L) inhibited the degradation of quinolone; the kinetics of quinoline and indole was followed the zero-order kinetics model; through GC-MS analyses, the intermediate metabolites of quinoline were 2-hydroxyquinoline and 2,8-dihydroxyquinoline; and metabolites of indole were oxindole and isatin. The high-throughput sequencing technology was used to analyze the microbial community structure, and results indicated that the functional bacterial were enriched in the anaerobic denitrification and methanogenesis system. The bacterial phylum was Proteobacteria, the dominant classes were Gammaproteobacteria and Betaproteobacteria, and the dominant genera were Acinetobacter, Candidimonas, Azospira, and Desulfomicrobium.更多还原