【摘要】 通过对中国严寒地区典型城市大庆市供暖季3类建筑（办公室、教室、住宅）室内外共计110个测点长期监测,分析大庆市供暖季室内外细颗粒物关联性,并基于16S rDNA基因测序技术和BLAST源解析技术研究大庆市供暖季3类建筑室内外细颗粒物上细菌的组分及来源.研究结果表明:大庆市供暖季室内外PM_2.5平均质量浓度分别为（32±22）和（45±34）μg/m³.其中办公室的平均渗透系数处于较低的状态（0.2886）,教室的渗透系数处于较高的状态（0.5702）,农村住宅（0.6513）比城市住宅的渗透系数略大（0.6057）.不同类型建筑室内细颗粒物中的细菌组分存在一定差异,室外细颗粒物中的细菌组分根据采样地点也存在不同,但整体上厚壁菌门（Firmicuts）、变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和生氧光细菌（Oxyphotobacteria）是大庆市供暖季细颗粒物中的优势菌群. 3类建筑室内外细颗粒物细菌来源主要为土壤、水体、人体、腐败有机物和粪便,但不同建筑类型及采样区域的细菌来源比例具有一定差异性.室内较室外人体来源所占比重大,而室外较室内土壤来源比重大.更多还原

【Abstract】 Through the long-term monitoring of indoor and outdoor fine particles of 3 building types（office, classroom and residence） for 110 samples in heating season of Daqing, a typical city in cold region of China, it analyzed the relationship between indoor and outdoor fine particles during heating season. Using 16 S rDNA gene analysis method and BLAST source apportionment, the components and sources of bacterial communities in fine particles of 3 building types indoors and outdoors were studied. The results showed that average mass concentrations of indoor and outdoor PM_2.5 in Daqing were（32±22） and（45±34）μg/m³, respectively during heating season. The average permeability coefficient of office was lower（0.2886）, classroom was higher（0.5702）, and rural residence（0.6513） was larger than urban residence（0.6057） slightly. Firmicuts, Proteobacteria, Bacteroidetes and Oxyphotobacteria were the dominant bacterial phyla in fine particulate matter of Daqing during heating season on the whole. The sources for 3 building types of bacteria in fine particulate matter indoors and outdoors were mainly soil, water, human, corrupt organic and faeces, while different building types and sampling sites led to different source proportions of bacteria.更多还原