【摘要】 选取中国南方地区典型生物尼罗罗非鱼作为受试生物,将其置于氯咪巴唑含量为2.00ng/mL的水体中暴露7d,考察氯咪巴唑在罗非鱼体内不同部位（鳃、肝脏、胆汁和血浆）的吸收和清除动力学过程,比较了氯咪巴唑在鱼体内不同部位的吸收和清除动力学速率.罗非鱼暴露于氯咪巴唑水溶液3d后,鱼体鳃、肝脏、胆汁和血浆中的氯咪巴唑达到最高浓度,分别为2.91ng/g,33.7ng/g,4.84ng/mL和2.58ng/mL;结束暴露3d后,氯咪巴唑在鳃、胆汁和血浆中的稳定浓度低于方法检测限,在肝脏中的稳定浓度为1.28ng/g.鱼体鳃、肝脏、胆汁和血浆中氯咪巴唑的吸收动力学常数k_u分别为0.069,0.813,0.286和0.136h^-1;清除动力学常数k_e分别为0.033,0.029,0.082和0.060h^-1;半衰期t_1/2分别为21.1,23.9,8.51和11.6h.氯咪巴唑在罗非鱼体内的吸收/清除动力学过程均符合伪一级动力学方程,方程的相关系数r²范围为0.75～0.98.氯咪巴唑在鱼体鳃、肝脏、胆汁和血浆中的稳态生物富集系数对数值logBCF_ss分别为0.50,1.56,0.72和0.45,低于氯咪巴唑在野生罗非鱼体内的富集系数.更多还原

【Abstract】 We used a typical fish species in South China, tilapia, to investigate the uptake and elimination kinetics of climbazole in fish gill, liver, bile and plasma following a 7-d exposure to aqueous solution of climbazole at the concentration of 2.00 ng/mL. We also compared the uptake and elimination rates of climbazole in fish tissues. In the uptake process, the climbazole concentration reached the maximum after 3 days of exposure, at which point the concentration in fish gill, liver, bile and plasma were up to 2.91 ng/g, 33.7 ng/g, 4.84 ng/mL and 2.58 ng/mL, respectively. In the elimination process, the climbazole was nearly eliminated in fish after 3 days, with the climbazole concentrations lower than MQLs in fish gill, bile and plasma, and of 1.21 ng/g in fish liver. The uptake kinetic constants（k_u） of climbazole in fish gill, liver, bile and plasma were 0.069, 0.813, 0.286 and 0.136 h^-1, respectively; while the corresponding elimination kinetic constants（k_e） were 0.033, 0.029, 0.082 and 0.060 h^-1, respectively. The half-time values of climbazole in fish gill, liver, bile and plasma were 21.1, 23.9, 8.51 and 11.6 h, respectively. The uptake and elimination processes of climbazole in fish tissues followed the pseudo first-order kinetic model, with the correlation coefficients r² in the range of 0.75～0.98. The log bioconcentration factor of climbazole at steady state in fish gill, liver, bile and plasma were 0.50, 1.56, 0.72 and 0.45, respectively, which were lower than those in wild tilapia fish.更多还原