【摘要】 为满足禽流感病毒高通量快速检测的需要,建立了一种能够检测各亚型禽流感病毒的实时荧光定量RT-PCR检测方法,并对该方法的特异性和灵敏度进行评估,使用该方法与农业行业标准NY/T772—2013中的禽流感病毒RT-PCR方法同时进行临床样品检测。结果显示,该方法检测耗时短、特异性好,检测下限达10-4 ng/μL,与传统的RT-PCR方法阳性符合率为100%。结果表明,本方法能实现对禽流感病毒的安全、特异、快速、灵敏、简单、高通量检测,从而弥补了现有传统检测技术的不足。更多还原

【Abstract】 In order to achieve the high-throughput and rapid detection of avian in?uenza virus(AIV),a real-time fluorescent quantitative RT-PCR method was established for detecting all subtypes of AIV. Then its specificity and sensitivity were evaluated,and some clinical samples were tested simultaneously by the established method and the conventional RT-PCR stipulated in the agricultural industry standard NY/T772—2013. Results showed that the detection limit of the established method could reach 10-4 ng/μL with short detection time and better speci?city,and the positive coincidence rate was 100% as compared with traditional RT-PCR method. In conclusion,the established real-time RTPCR method was safe,speci?c,rapid,sensitive and simple,and could achieve the high-throughput detection for AIV,which covered the de?ciency of current traditional detection technologies.更多还原