【摘要】 目的:将微生物电解池(MEC)与厌氧膜生物反应器(AnM BR)耦合,构建MEC-AnM BR系统,以期同步实现污水高效处理和膜污染缓解,推动膜生物反应器的理论创新和技术创新。创新点:1.将MEC与AnM BR耦合,构建MEC-AnM BR系统用于高浓度有机废水的处理;2.研究反应器运行和微生物群落之间的关系;3.探究膜污染运行周期中各膜污染阶段微生物代谢产物与自身代谢活性的变化规律。方法:1.启动和运行MEC-AnM BR反应器,并与传统AnM BR对照,综合考察MEC-AnM BR反应器的运行性能; 2.利用高通量测序技术对传统AnM BR和MEC-AnMBR各膜污染阶段的阴极膜表面微生物群落结构及多样性进行研究,并综合分析MEC-AnMBR反应器的运行特性与微生物群落间的相互关系;3.对MEC-AnM BR反应器阴极膜组件及微生物分泌物进行原位观察,并研究其在膜污染运行周期中各膜污染阶段微生物代谢产物与自身代谢活性的变化规律。结论:1.成功构建微生物电解池MEC-AnMBR生物系统;2.与AnM BR相比,MEC-AnM BR中的化学需氧量(COD)去除效率和甲烷产量分别增加6.7%和77.1%;3.与AnM BR相比,MEC-AnM BR的膜污染因细胞外聚合物和可溶性微生物产物增长缓慢而大大减少;4.高通量测序分析表明MEC-AnM BR富含互养菌属(Synergistaceaeuncultured)和互营热菌属(Thermovirga),而Thermovirga是关键的功能性微生物;5.这些结果表明MEC-AnMBR可同时提高反应器效率并减轻膜污染。更多还原

【Abstract】 Membrane fouling restricts the wide application of anaerobic membrane bio-reactors(AnMBRs). In this study, a microbial electrolytic cell(MEC)-AnMBR biosystem was constructed to relieve membrane fouling. Total chemical oxygen demand(COD) removal efficiency and methane production in MEC-AnM BR were increased to 6.7% and 77.1%, respectively, in comparison to AnM BR. The membrane fouling of MEC-AnMBR was greatly lessened by the slower growth of extracellular polymeric substances(EPS) and soluble microbial products(SMP). High-throughput sequencing analysis showed that Synergistaceae-uncultured and Thermovirga were enriched in MEC-AnM BR, and Thermovirga was found as the key functional microorganism. These results indicated that MEC-AnMBR could simultaneously enhance the reactor efficiency and mitigate membrane fouling.更多还原