【摘要】 目的从白及Bletillastriata块茎中分离纯化多糖BSP-1,并对其结构和抗肿瘤活性进行研究。方法将提取的粗多糖经DEAE-cellulose柱色谱分离得到3个多糖组分BP-1、BP-2、BP-3。BP-1通过SephadexG-200柱色谱纯化后得到组分BSP-1。采用HPGPC、IR、GC、GC-MS、甲基化及¹H-、¹³C-NMR等方法对该多糖的结构进行表征,并考察BSP-1抗肿瘤活性。结果测得BSP-1相对分子质量为4.72×10⁵,由葡萄糖和甘露糖组成。主链由β-1,4甘露糖,侧链由末端连接的葡萄糖组成,其物质的量比为8∶1。体外肿瘤细胞活性实验表明,BSP-1能抑制肝癌Hep G2细胞的增殖。BSP-1体内可显著抑制裸鼠的瘤质量,抑制率为66.42%。结论 BSP-1的结构确定为进一步阐明和开发白及中多糖类成分提供了一定的参考。更多还原

【Abstract】 Objective Bletilla striata polysaccharide（BSP-1） extracted from the stem tubers of B. striata was purified to study the structural properties and antitumor activity. Methods The crude polysaccharide was fractionated by DEAE-cellulose column chromatography, and three fractions were obtained including BP-1, BP-2, and BP-3, respectively, BP-1 was further purified by SephadexG-200 column chromatography, and a sub-fraction named BSP-1 was obtained. Subsequently, HPGPC, IR spectroscopy, gas chromatography（GC）, GC coupled to mass spectrometry（GC-MS） and methylation analysis, and ¹ H-and ¹³ C-NMR were employed to characterize the structural properties of the polysaccharide fractions. Moreover, the anticancer activity was investigated in vivo. Results The results showed that the molecular weight of BSP-1 were 4.72 × 10⁵. BSP-1 was consisted of mannose（Man） and glucose（Glc） residues. The backbone of BSP-1 was composed of β-1,4-linked Man and Glc residues at the end with a molar ratio of 8∶1. BSP-1 could inhibit the tumor proliferation of HepG2-bearing mice. The tumor weight of mice was significantly inhibited by BSP-1 with inhibitory rate of 66.42%. Conclusion Structural characterization of BSP-1 provides the significant reference for the further development and elucidation of polysaccharides from B. striata as new drugs.更多还原