【摘要】 克隆红肉苹果‘红心16号’果实的MdMYB111,原核诱导并获得其重组蛋白,对MdMYB111进行生物信息学分析,测定其在果肉色泽差异明显的品种‘红心16号’和‘红脆1号’果实中的表达水平,并通过转基因鉴定其功能。结果表明,MdMYB111的开放阅读框为720 bp,编码239个氨基酸,预测其蛋白大小为26.93 kD;进化树分析发现MdMYB111和MdMYB16在同一个进化支上,且其蛋白C端存在EAR抑制序列;‘红心16号’果实成熟期果肉全部呈现红色,并且颜色较深;‘红脆1号’果实成熟期果肉呈现浅红色,红色范围较小。‘红心16号’果实花青苷含量和MdANS、MdUFGT、MdMYB9、MdMYB10、MdMYB11的表达水平均高于‘红脆1号’,但MdMYB16和MdMYB111的表达水平低于‘红脆1号’;在新疆红肉苹果愈伤中过表达MdMYB111,能够抑制MdANS和MdUFGT的表达并降低花青苷的含量。综上所述,MdMYB111可能参与花青苷合成途径并抑制其合成。更多还原

【Abstract】 The fruit of‘Hongxin 16’apple,a progeny of Malus sieversii f. neidzwetzkyana hybrids,was used as the test material. MdMYB111 was cloned and its recombinant protein was induced and expressed in prokaryotic analysis. The bioinformatic analysis of this gene was conducted,its expression level was determined in the fruits of the varieties of‘Hongxin 16’and‘Hongcui 1’and its function was identified by transgene. The results showed that the open reading frame of MdMYB111 was 720 bp,encoding 239 amino acids,and its predicted protein size was 26.93 kD. Phylogenetic tree analysis showed that MdMYB111 and MdMYB16 were in the same clade and there was an EAR inhibitory sequence at the C-terminus of the protein. The ripened fruit of‘Hongxin 16’ is complete red and darker in color. The fruit of‘Hongcui 1’has a light red color and a small red range. On the‘Hongxin 16’,both the content of anthocyanin in fruits mature stage and MdANS,MdUFGT,MdMYB9,MdMYB10,MdMYB11 expression levels were higher than that of‘Hongcui 1’,but the expression levels of MdMYB16 and MdMYB111 were lower than‘Hongcui 1’. Overexpression of MdMYB111 in red apple callus can inhibit the expression of MdANS and MdUFGT and reduce the content of anthocyanin. In summary,MdMYB111 may be involved in the anthocyanin synthesis pathway and inhibit its synthesis.更多还原