【摘要】 肠道微生物对大熊猫的消化吸收和代谢免疫具有重要的意义.为了解上海、四川两地成体大熊猫肠道微生物结构的差异及其与消化纤维素能力的关系,对两地大熊猫肠道微生物多样性、纤维素酶活性以及两地环境差异、食物性质等进行分析,并对成体大熊猫肠道微生物性质与纤维素酶活性进行相关性分析.结果表明,上海成体大熊猫肠道菌群结构较四川成体大熊猫更加丰富,两地成体大熊猫肠道优势菌和特异性菌有较大差异.上海成体大熊猫具有更高的纤维素消化能力,这可能得益于其肠道中具有更丰富的细菌多样性和较丰富的芽孢杆菌属(Bacillus)及山野壳菌科未知菌属(Unclassified_f_Montagnulaceae).两地成体大熊猫肠道微生物多样性及消化纤维素能力的差异,可能是由食物性质、海拔等外在环境因素导致的.总体而言,不同地区成体大熊猫肠道微生物结构差异明显,且对大熊猫肠道的消化能力具有一定的影响.(图3表7参37)更多还原

【Abstract】 The giant panda is one of the rarest species in our country. People focus on raising, breeding, and conservation of giant pandas. The intestinal microorganisms are very important for giant pandas’ digestion, absorption, metabolism, and immunity. The purpose of this study was to investigate the differences in the intestinal microbial structure of adult giant pandas between Shanghai and Sichuan and the relationship with their ability to digest cellulose. The gut microbial diversity and cellulase activity of giant pandas, as well as the environmental differences and food properties in two places were analyzed.The correlation of the microbial properties and cellulase activities of adult giant pandas were analyzed. The microbial diversity was greater in the gut of adult giant pandas in Shanghai, and the dominant bacteria and unique genus of giant pandas in Shanghai were vastly different than that in Sichuan pandas. Moreover, the cellulose digestibility was higher in Shanghai because of its richer bacterial diversity and more abundant Bacillus and Unclassified_f_Montagnulaceae. The differences in the intestinal microbial structure and cellulose digestive abilities of adult giant pandas might be affected by some external environmental factors, such as food and attitude. Overall, the difference in the intestine microbial diversity of adult giant pandas in different regions could partly affect cellulolytic capacity.更多还原