【摘要】 对从中药白及新鲜药材中分离得到的一株内生菌草酸青霉菌Penicillium oxalicum进行固体发酵40 d后得到发酵产物,将发酵产物采用75%乙醇渗漉提取后,用乙酸乙酯萃取,并利用硅胶柱色谱、Sephadex LH-20凝胶、HPLC等方法进行成分分离.从中分离得到15个单体化合物,经质谱、核磁共振等波谱方法鉴定为α-亚麻酸甲酯(1)、亚油酸甲酯(2)、benzoic acid(3)、4-hydroxybenzaldehyde(4)、ganodermasides D(5)、dankasterone A(6)、calvasterol B(7)、cyclo-(Pro-Phe)(8)、penioxalicin(9)、5α-麦角甾-7,22-二烯-3β,5,6β-三醇(10)、cis-4-hydroxyscytalone(11)、3,4-dihydroxyphenylaceticacidmethylester(12)、protocatechuicacid(13)、cyclo-(4-S-hydroxy-R-proline-R-isoleucine)(14)、cyclo-L-(4-hydroxyprolinyl)-L-leucine(15).其中化合物1以及4-15均为首次从P. oxalicum中分离得到.本研究结果可促进白及及其内生菌资源的开发利用.(图1参32)更多还原

【Abstract】 To study the isolation and elucidation of chemical constituents of Penicillium oxalicum, an endophytic fungus was isolated from Bletilla striata(Thunb.) Reichb. f. After solid state fermentation for 40 days, secondary metabolites were percolated by ethanolwater(75:25, V/V), extracted with ethyl acetate, and purified by column chromatography methods including silica gel, Sephadex LH-20, and high-performance liquid chromatography. A total of 15 compounds were isolated. Their structures were elucidated through extensive spectroscopic analyses as follows: α-methyl linolenic acid(1), methyl linoleate(2),benzoic acid(3), 4-hydroxybenzaldehyde(4), ganodermasides D(5), dankasterone A(6), calvasterol B(7), cyclo-(Pro-Phe)(8),penioxalicin(9), 5α-ergoside-7,22-diene-3β,5,6β-triol(10), cis-4-hydroxyscytalone(11), 3,4-dihydroxyphenylacetic acid methyl ester(12), protocatechuic acid(13), cyclo-(4-S-hydroxy-R-proline-R-isoleucine)(14), and cyclo-L-(4-hydroxyprolinyl)-L-leucine(15). Among these, compounds 1 and 4-15 were isolated from P. oxalicum for the first time.更多还原