【摘要】 为研究不同木槿品种的花粉生活力及柱头可授性强弱,以9个木槿品种为试验材料,采用TTC染色法、I₂-KI染色法及联苯胺-甲萘酚染色法3种染色方法测木槿新鲜花粉的活力,并用联苯胺-甲萘酚染色法测定不同贮藏条件下木槿各品种的花粉生活力;用联苯胺-过氧化氢法测定木槿各品种的柱头可授性。结果表明:TTC染色法不适用于检测木槿花粉活力,I₂-KI染色法及联苯胺-甲萘酚染色法适用于检测木槿花粉活力。2种染色法测得的‘白边红心’的生活力都最高,I₂-KI染色法的生活力为53.48%,联苯胺-甲萘酚染色法的生活力为90.09%。‘牡丹木槿’的生活力最低,I₂-KI染色法的生活力为4.38%,联苯胺-甲萘酚染色法的生活力为42.96%。不同品种在3种贮藏温度下花粉生活力变化趋势相同,4℃条件下有利于延长花粉的贮藏时间。‘牡丹木槿’的柱头可授性最弱。更多还原

【Abstract】 To investigate the pollen viability and stigma receptivity of different Hibiscus syriacus cultivars,9 cultivars were used as experimental materials,fresh pollen viability was measured by TTC coloration,I₂-KI staining and benzidine-naphthol staining.Pollen viability of different varieties under different storage conditions was determined by benzidine-methylnaphthol staining.Stigma receptivity of different varieties was determined by benzidine-hydrogen peroxide method.The results indicated that TTC staining was not suitable for the detection of Hibiscus syriacus’s pollen viability,while I₂-KI staining and benzidine-cresol staining were suitable for the detection.The viability of ‘Red Heart’ was the highest,the viability by I₂-KI staining method was 53.48% and that of benzidine-cresol staining method was 90.09%.The viablility of ‘Mudan’ was the lowest,the viability by I₂-KI staining method was 4.38% and that of benzidine-cresol staining method was 42.96%.The changing trends of pollen viability were similar under three storage temperature for different cultivars and the most proper temperature for pollen storage was 4℃.The stigma receptivity of ‘Mudan’ variety was the lowest.更多还原