【摘要】 目的建立适宜的复合扩增体系,并在中国东北汉族人群中验证所选择的DIP-STR遗传标记,以推动DIP-STR遗传标记应用于实际办案。方法参照Diana Hall等人设计的单遗传标记扩增体系,建立合适的复合扩增体系并以中国东北汉族人群进行验证,对等位基因频率、单倍型数、观察杂合度、有效基因型等相关参数数据进行统计和计算。结果构建了合适的复合扩增体系。经对107个样本验证估计得出等位基因频率,所选择8个DIP-STR遗传标记具有较高的有效基因型概率(I值)。结论 8个DIP-STR遗传标记在中国东北汉族人群中具有应用价值。更多还原

【Abstract】 Objective Establishing an appropriate composite amplification system of DIP-STR genetic markers to promote their application into practical cases through verifying them in the Han population of northeast China. Methods Based on the single-genetic marker amplification system designed by Diana Hall et al, a suitable composite amplification system of DIP-STR genetic markers was to build up and verify in the Han population of northeast China. Statistical analysis and calculation were carried out into the relevant data of allelic frequency, haplotype amount, observed heterozygosity and informative genotypes.Results The composite amplification system of DIP-STR genetic markers was successfully established. With the estimation of allelic frequencies through verification from 107 samples into the established composite amplification system, the selected 8 DIPSTR genetic markers had a high probability of informative genotype(I value). Conclusion The eight DIP-STR genetic markers and the involving composite amplification system have forensic applicability in the Han population of northeast China.更多还原