【摘要】 [目的]主要探究内生真菌Acremoniumsp.D212(枝顶孢霉D212)与植物激素水杨酸相互作用调控植物发育的机制。[方法]用枝顶孢霉D212与三七共培养后,通过转录组测序及PCR方法,检测基因表达;将枝顶孢霉D212接种水稻培养后,进行免疫反应,观察枝顶孢霉D212在水稻根中的定殖及水稻根系的发育情况;利用免疫组化及细胞学的方法观察生长素输出蛋白的细胞定位。[结果]枝顶孢霉D212与三七共生时,引起了水杨酸途径及病程防御相关基因的大量表达,但没诱导病程防御反应基因表达水平的提高。但是,降低了独脚金内酯中PnDAD2d,PnD14c及PnD27基因的表达。枝顶孢霉D212可以同水稻共生,并可大量定殖于水杨酸受体突变体OsNPR1-RNAi植株中,促进根系的生长。枝顶孢霉D212与水稻OsNPR1-RNAi突变体的共生降低了生长素输出蛋白OsPIN1与OsPIN2在根表皮细胞中的细胞膜定位。[结论]枝顶孢霉D212与宿主植物共生需要水杨酸受体NPR1,并改变生长素的运输而影响根系生长。更多还原

【Abstract】 [Objective]This study aimed to detect the mechanism of endophytic fungus Acremonium sp.D212 and phytohormone salicylic acid interaction in regulating plant development.[Method]The genes expression levels were detected by transcriptome sequencing and PCR method after Panax notoginseng inoculated with Acremonium sp.D212 The colonization of Acremonium sp.D212 and rice root development were observed,after rice inoculated with Acremonium sp.D212.Immunohistochemical and cytological methods were used to detect the cellular localization of auxin efflux protein.[Result]Symbiosis of Acremonium sp.D212 and P.notoginseng induced gene expression,and most of these genes involved in salicylic acid pathway and defence reaction,but did not increased the expression levels of pathogenesis-related genes.However,the expression levels of strigolactone genes PnDAD2 d,PnD14 c and PnD27 were decreased.Acremonium sp.D212 could form symbiont with rice and effectively colonized in salicylic acid receptor mutant OsNPR1-RNAi to promote root growth.The localization of auxin efflux protein OsPIN1 and OsPIN2 in plasmamembrane of root epidermal cell of rice mutant OsNPR1-RNAi inoculated with Acremonium sp.D212 were decreased.[Conclusion]The salicylic acid receptor NPR1 was required for the symbiosis of Acremonium sp.D212 and host plant in regulating root growth by disruption of auxin transport.更多还原