【摘要】 选取黔中地区的贵阳市乌当区百宜乡(WD)、花溪区孟关(MG)和贵州省黔南龙里县龙架山森林公园(LL)3个地区的马尾松-杜鹃群落为研究对象,采集杜鹃根围土壤进行高通量测序,分析物种多样性,并通过数量生态学原理分析植物群落物种α多样性及土壤养分对真菌群落的影响.结果表明:从杜鹃的根围土壤中获得有效序列505 444条,隶属于9门23纲84目169科296属真菌(3 435 OTU),其中LL有真菌9门19纲53目90科127属(495 OTU)、 WD有8门21纲78目150科246属(1 076 OTU)、 MG有6门17纲55目99科129属(522 OTU).菌群α多样性分析显示, Chao1,ACE,Shannon指数均表现为WD大于MG和LL.菌群群落结构受生境植物多样性、土壤养分的综合作用,土壤有机质、 pH值以及速效磷、速效氮、全氮含量较大程度解释了真菌对土壤养分变化的响应.更多还原

【Abstract】 Taking the Pinus massoniana-Rhododendron simsii communities in three areas of WuDang(WD), MengGuan(MG) and LongLi(LL) in Central Guizhou as the research objects, the rhizosphere soil of R. simsii was collected for high-throughput sequencing to analyze species diversity. The effects of vegetation α diversity and soil nutrient on fungal communities were analyzed with the principle tative ecology. A total of 505 444 effective sequences were obtained from the rhizosphere soil of R. simsii, belonging to 9 phyla, 23 classes, 84 orders, 169 families and 296 genera(3 435 OTU). Among them, LL fungi 9 phyla, 19 classes, 53 orders, 90 families, and 127 genera(495 OTU); WD fungi 8 phyla, 21 classes, 78 orders, 150 families and 246 genera(1 076 OTU); and MG fungi 6 phyla, 17 classes, 55 orders, 99 families and 129 genera(522 OTU). Alpha diversity analysis showed that the Chao1, ACE and Shannon indices were all in the order of WD>MG and LL. The community structure of the flora was affected by the diversity of habitat plants and soil nutrients, and fungi diversity was consistent with plant diversity. Soil organic matter(C), pH, available phosphorus(AP), available nitrogen(AN), and total nitrogen(TN) largely explain the response mechanism of the distribution of fungi species to the changes of soil nutrient.更多还原