【摘要】 【目的】研究聚对苯二甲酸-己二酸丁二酯(Poly(butylene adipate-co-terephthalate),PBAT)生物降解膜对土壤理化性质及氮循环相关微生物的影响。【方法】以铺覆PE膜为对照,利用实时荧光定量PCR和末端限制性片段长度多态性(T-RFLP)技术解析铺覆PBAT生物降解膜中土壤氮循环相关微生物的数量和群落多样性。【结果】同种作物(除棉花外)铺覆PE膜和PBAT生物降解膜土壤理化性质差异不显著。同种作物铺覆PBAT生物降解膜与PE膜之间,AOA-amoA、AOB-amoA及nosZ基因丰度无显著差异,而不同作物对AOA-amoA、AOB-amoA及nosZ基因丰度的影响较为明显。【结论】PE膜和PBAT生物降解膜对土壤理化性质及氮循环微生物的影响差异不显著, PBAT生物降解膜可以作为PE膜的替代品以解决PE膜造成的白色污染。更多还原

【Abstract】 【Objective】 To study the effect of Poly(butylene adipate-co-terephthalate) biodegradation membrane on soil physicochemical properties and nitrogen-cycle-related microorganisms. 【Method】Compared with coated PE membrane, the number and community diversity of soil nitrogen cycle related microorganisms in coated PBAT biodegradable membrane were analyzed by real-time fluorescence quantitative PCR and terminal restriction fragment length polymorphism(T-RFLP). 【Result】There was no significant difference in soil physical and chemical properties of the same crops(except cotton) between PE membrane and PBAT biodegradable membrane. There was no significant difference of AOA-amoA, AOB-amoA and nosZ gene abundance between the same crops covered with PBAT biodegradable membrane and PE membrane, while the difference of AOA-amoA, AOB-amoA and nosZ gene abundance was significant for the different crops. 【Conclusion】There was no significant difference between the effects of PE membrane and PBAT biodegradable membrane on soil physical and chemical properties and nitrogen cycling microorganisms. PBAT biodegradable membrane can be used as a substitute for PE membrane to solve the white pollution caused by PE membrane.更多还原