【摘要】 【目的】揭示乌鲁木齐地震断裂带泉水中古菌群落对水文地球化学元素变化的响应。【方法】采用微孔滤膜法收集泉水中菌体,直接提取环境总DNA,嵌套式PCR扩增古菌16S rDNA基因V3区,变性梯度凝胶电泳(DGGE)检测古菌群落结构的变化,所得信息与监测的地球化学指标进行典型相关分析。【结果】DGGE分析及切胶测序共得到古菌14个主要类群,分属于广域古菌和泉古菌两个门,全部为不可培养类群,其中前者占绝对优势。典型相关性分析发现类群B-11和B-12与He以及CH4含量呈正相关,与硫化物呈负相关;类群B-2、B-3和B-9与F-离子含量成正相关而类群B-13与它呈负相关;而仅有的泉古菌B-7与CH4呈负相关。另外,所得绝大部分古菌类群与其它冷泉或者低温环境中发现的古菌类群具有95%左右的相似性。【结论】乌鲁木齐断裂带泉水古菌群落能够对泉水中的某些地球化学元素变化产生一定的响应,并且可能存在大量嗜冷新类群。更多还原

【Abstract】 【Objective】The study was to reveal the archaeal community response to the hydrogeochemical element changes in the spring water at seismic fault belt in Urumqi.【Method】Microbial communities were collected by microporous membrane filtration,then total DNA were directly extracted by enzymatic digestion and the V3 region of the archaeal 16S rDNA gene was amplified by Nested PCR.The change of archaeal community structure was detected by denatured gradient gel electrophoresis(DGGE) analysis,the correlations between hydrogeochemical parameters and obtained information were analyzed by canonical correspondence analysis(CCA).【Result】Fourteen major bands were obtained from DGGE gels and used for direct sequencing.BLAST analysis showed that all of them were uncultured archaea and phylogenetic analysis classified them into phyla Euryarchaeota and Crenarchaeota,and the former was dominating.CCA revealed that groups B-11 and B-12 were positively correlated to helium and methane,and negatively correlated to sulfide;groups B2,B-3 and B-9 were positively correlated to F-,while,the group B-13 was the opposite;the only Crenarchaeota archaeal B-7 was negatively correlated to methane.【Conclusion】The results suggest that archaeal communities in seismic fault belt spring water could response to some hydrogeochemical parameter changes and there maybe existence of large number of novel psychrophilic groups.更多还原