【摘要】 [目的]了解汶川大地震后某板房社区内流行性脑膜炎奈瑟菌(Neisseria meningitides,Nm)带菌率状况和主要流行的血清群,为评价灾区卫生防疫效果和调整卫生防疫措施提供依据。[方法]对灾区某板房社区人群进行单纯随机抽样,采集咽拭子样品107份,采用传统的细菌学分离培养方法和分子生物学Taqman-MGB探针RT-PCR技术对流脑带菌率进行检测;对分离所得菌株进行血清学鉴定和PCR方法分群。[结果]对所采集的107份样品采用分子生物学Taqman-MGB探针RT-PCR技术检出3例阳性;细菌学分离培养得到3株阳性菌株,两种检测方法阳性样品编号一致,检测结果吻合,阳性率为2.80%。3株阳性菌株经血清学和分子生物学PCR方法分群,分别为B群Nm2株,C群Nm1株。[结论]与四川省流脑检测点及全国其他地区流脑带菌水平相比,社区流脑带菌率没有明显差别,流行仍以B群为主,但是流脑C群的出现应该引起足够的重视,防止流脑疫情的出现。更多还原

【Abstract】 [Objective] To understand the Neissria meningitides-carrying rate and major epidemic serogroups in one prefab community of disaster area. To provide basis for evaluating the effects of epidemic prevention and adjustment of health and epidemic prevention measures for disaster area. [Methods] 107 throat swabs were collected from one prefab community of disaster area, used the traditional isolation and culture method and TaqMan assay for detection. The isolated strains were indentified by serotyping of and grouped by PCR. [Results] 3 positive samples were identified by the traditional method and TaqMan assay. Results were consistent with the two detection methods, the positive rate was 2.80%. 2 strains were Group B and 1 was Group C. [Conclusion] Comparing with epidemic cerebrospinal meningitis detection point in Sichuan Province and other parts of China, the germ-carrying rate is not high enough in the prefab community of disaster area. Group B is still the main epidemic flora. But we should pay attention to the emergence of Group C to prevent the outbreak of epidemic cerebrospinal meningitis.更多还原