【摘要】 目的探讨程序性死亡受体-1(programmed death receptor-1,PD-1)、程序性死亡配体-1(programmed death receptor ligand-1,PD-L1)、癌胚抗原125(carcinoembryonic antigen 125,CA125)在原发性肝癌中的表达变化及临床意义。方法选取2016年3月至2018年3月在我院就诊的原发性肝癌患者70例作为研究对象。取所有患者手术切除癌组织标本以及与距离癌组织5 cm以上的癌旁正常肝组织,进行连续切片作免疫组化标记以及革兰染色待用。酶联免疫吸附测定PD-1、PD-L1、CA125表达,免疫组化染色处理后进行结果判定。结果原发性肝癌组织CA125、PD-1、PD-L1阳性表达水平显著高于癌旁组织(P <0. 05)。原发性肝癌组织PD-1、PD-L1、CA125阳性表达与患者TNM分期、肿瘤大小、肿瘤转移、腹水有关。Ⅲ-Ⅳ期、肿瘤> 5cm、有肿瘤转移、有腹水的患者癌组织PD-1、PD-L1、CA125阳性表达率均较高(P <0. 05)。原发性肝癌组织PD-1、PD-L1、CA125两两之间呈正相关(P均<0. 05)。结论 PD-1、PD-L1、CA125表达水平与原发性肝癌具有相关性,PD-1、PD-L1、CA125的异常表达,参与了原发性肝癌的发展,为原发性肝癌的治疗提供一定的理论基础。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of programmed death receptor-1( PD-1),programmed death ligand-1( PD-L1) and carcinoembryonic antigen 125( CA125) in primary liver cancer. Methods Seventy patients with primary hepatocellular carcinoma treated in our hospital from March 2016 to March 2018 were selected as the study subjects. All patients met the diagnostic criteria of primary liver cancer. All specimens of cancer tissues and normal liver tissues adjacent to the cancer tissues more than 5 cm away from the cancer tissues were taken for immunohistochemical staining and Gram staining. The expressions of PD-1,PD-L1 and CA125 were detected by ELISA,and the results were determined by immunohistochemical staining.Results The positive expression levels of CA125,PD-1 and PD-L1 in cancer tissues were significantly higher than those in adjacent tissues( P < 0. 05). The positive expression of PD-1,PD-L1 and CA125 was related to TNM stage,tumor size,tumor metastasis and ascites in patients with primary hepatocellular carcinoma. The positive expressions of PD-1,PD-L1 and CA125 were higher in patients with ascites( P < 0. 05). There was a positive correlation between PD-1 and PD-L1,between PD-1 and CA125,and between PD-L1 and CEA125. Conclusion The expressions of PD-1,PD-L1 and CA125 are correlated with primary hepatocellular carcinoma. The abnormal expressions of PD-1,PD-L1 and CA125 participate in the development of primary hepatocellular carcinoma and provide a theoretical basis for the treatment of primary hepatocellular carcinoma.更多还原