【摘要】 2018年7月,山东省某育肥猪场猪群突然发病并出现大量死亡,病猪临床表现高热、皮肤潮红并布满点状出血。为查明发病原因,对病猪进行病理剖检并采样,经实验室综合检测,初步诊断为猪瘟病毒感染。对检测到的CSFV E2基因进行了分子测序及核酸序列同源性对比,最终确诊为2.1d亚型猪瘟病毒感染。结果显示,本实验室分离到的CSFV(命名为SDXT2018)与21株参考毒株的E2基因核苷酸序列同源性为81.3%～96.2%。与21株参考毒株中同源性最高的是2.1d亚型的JQ001834,同源性达96.2%;与2.3亚型的经典毒株HQ148061的同源性最低,为81.3%。结果说明,我国猪瘟病毒流行毒株的变异趋势越发严峻,以致于常规疫苗免疫的猪场有可能爆发典型猪瘟疫情,这对养猪业构成了严重威胁。因此,亟待人们开展病毒遗传变异趋势的研究,并针对病毒抗原特性研制、生产高效疫苗。更多还原

【Abstract】 In July 2018, a deathful disease outbroke in a fattening pigs farm in Shandong province. Clinical symptoms included high fever, flushed skin with dotted bleeding. It was preliminarily diagnosed as Classical Swine Fever(CSF) after autopsy and a comprehensive laboratory detection. Then, the E2 gene of Classical Swine Fever virus(CSFV) was detected and sequenced. This outbreak was confirmed as infection of CSFV. The nucleotide sequence homology between the strain in this study(named SDXT2018) and reference strains was from 81.3% to96.2%. In reference strains, SDXT2018 was closest to JQ001834 that belongings to 2.1 d subtype. The homology between SDXT2018 and AF091507, which was classical attenuated hog fever vaccine strain, was just 81.3%. The results showed variation of the epidemic strains of CSFV in China is becoming severe, which seriously threat the swine industry. Classical Swine Fever could outbreak in immunity-failure farms and cause enormous economic loss. It is urgent to research the trend of viral variation and produce new efficient vaccine.更多还原