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谷子苗期耐低磷种质筛选及其根系保护酶系统对低磷胁迫的响应

Screening of germplasm tolerant to low phosphorus of seedling stage and response of root protective enzymes to low phosphorus in foxtail millet

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【作者】 苑乂川陈小雨李明明李萍贾亚涛韩渊怀邢国芳

【Author】 YUAN Yi-Chuan;CHEN Xiao-Yu;LI Ming-Ming;LI Ping;JIA Ya-Tao;HAN Yuan-Huai;XING Guo-Fang;College of Agronomy,Shanxi Agricultural University;Institute of Agricultural Bioengineering,Shanxi Agricultural University;Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crop;

【通讯作者】 邢国芳;

【机构】 山西农业大学农学院山西农业大学农业生物工程研究所杂粮种质资源发掘与遗传改良山西省重点实验室

【摘要】 本研究旨在探讨不同基因型谷子苗期耐低磷特性,建立其筛选的评价体系,筛选出苗期耐低磷谷子种质材料。对160份核心谷子种质资源的苗期株高、根长、地上鲜重、地下鲜重、叶长、叶宽、茎粗、地上磷含量、地下磷含量、地上干重和地下干重11个指标的耐低磷系数进行变异分析、相关性分析、主成分分析和聚类分析,采用隶属函数法综合评价不同谷子耐低磷相关指标和耐低磷特性;针对筛选出的低磷敏感和耐低磷品种,在生理水平上分析其根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性。结果表明, 9份材料属于低磷敏感品种, 66份材料属于低磷较敏感品种, 70份材料属于较耐低磷品种, 15份材料属于耐低磷品种;低磷胁迫促使谷子根系SOD、POD、CAT活性增加。总之,以综合指标对谷子耐低磷特性的鉴定更为客观;根系保护酶系统对谷子在低磷胁迫下的适应性具有重要作用。

【Abstract】 The purpose of this paper was to explore the characteristics of low-phosphate tolerance in different genotypes of foxtail millet and establish an evaluation system for screening and breeding new cultivars with low-phosphate tolerance.Correlation analysis,principal component analysis,membership function and cluster analysis were carried out on 11 indicators of 160 core foxtail millet varieties,including plant heights,root lengths,fresh weights of aerial part and underground part,leaf lengths and widths,stem thicknesses,phosphorus contents of aerial part and underground part,dry weights of aerial part and underground part,to comprehensively evaluate indicators and characters related to low-phosphorous tolerance.Furthermore,a hydroponic experiment was conducted to cultivate selected varieties with low-phosphorus sensitivity and low-phosphorus tolerance under low phosphorus(LP) and normal phosphorus(NP) conditions,and superoxide dismutase(SOD) activity,peroxidase(POD) activity,and catalase(CAT) activity in roots were analyzed.There were nine varieties with low-phosphorus sensitivity,66 and 70 varieties with medium low-phosphate tolerance and 15 varieties with low-phosphorus tolerance.The activities of SOD,POD,and CAT were increased under low phosphorus condition.It was concluded that the comprehensive index is more objective to evaluate the low-phosphorus tolerance of 160 foxtail millet variation at the seedling stage,and the root protective enzymes are important for the adaptability of foxtail millet to low-phosphorus stress.

【基金】 国家自然科学基金项目(31200914,31501323);山西省高等学校科技创新项目(2015145);山西省重点研发计划项目(201803D221008-4);山西省主要农作物种质创新与分子育种重点科技创新平台(201605D151002)资助~~
  • 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,2019年04期
  • 【分类号】S515
  • 【网络出版时间】2019-01-04 16:46
  • 【被引频次】13
  • 【下载频次】481
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