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耐镉甲基营养芽胞杆菌吸附条件的优化及其抗逆性

Optimization of cadmium adsorption conditions and anti-stress characteristics of a cadmium-tolerant Bacillus methylotrophicus strain

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【作者】 张冬雪文亚雄罗志威李雪玲郑双凤谭石勇

【Author】 Dongxue Zhang;Yaxiong Wen;Zhiwei Luo;Xueling Li;Shuangfeng Zheng;Shiyong Tan;Key Laboratory of Plant Nutrition and Biological Fertilizer, Ministry of Agriculture;Efficient Utilization of Heavy Metal Contaminated Farmland,Engineering Research Center in Hunan Province;Biofertilizer Engineering & Technology Research Center in Hunan Province;Taigu Ecological Biotechnology Group Limited Company;

【通讯作者】 谭石勇;

【机构】 农业部植物营养与生物肥料重点实验室重金属污染耕地安全高效利用湖南省工程研究中心湖南省生物肥料工程技术研究中心泰谷生态科技集团股份有限公司

【摘要】 【目的】对所筛选的1株耐镉甲基营养芽胞杆菌NTGB29进行了环境抗逆性的研究,及影响菌株吸附镉离子效率的条件优化。【方法】以发酵液活菌数为指标,研究其对不同NaCl浓度、酸碱度、镉离子浓度的耐受情况;进一步通过单因素实验和响应面法优化影响菌株镉离子吸附效率的发酵条件;以有效镉离子含量为指标,验证菌株在镉污染土壤中的吸附效果。【结果】结果表明,菌株NTGB29对NaCl浓度、酸碱度、Cd2+浓度的最大耐受值分别为10%、pH11.0、50mg/L;菌株在发酵液初始Cd2+浓度10 mg/L、起始pH 6.4、培养温度37°C、NaCl浓度4.2%、装液量50 mL/250 mL、培养时间24 h时,对Cd2+的吸附率达到79.70%;菌株能有效降低镉污染土壤中的有效镉离子含量,吸附率为29.65%。【结论】菌株NTGB29在较高浓度Cd2+浓度、NaCl浓度及强碱环境条件下仍然能够生长,具有良好的环境抗逆性及Cd2+吸附能力,在镉污染土壤调理剂及微生物功能菌剂的研制方面能够提供有价值的菌种资源。

【Abstract】 [Objective] To assess the anti-stress characteristics and to optimize the conditions affecting its cadmium ions adsorption efficiency of a cadmium-tolerant Bacillus methylotrophicus strain NTGB29. [Methods] The anti-stress characteristics were studied by plate colony-counting method. In addition, we used single factor experiment and response surface methodology to optimize the fermentation conditions of cadmium ions adsorption of the strain. Moreover, the available cadmium ions contents were used to verify the cadmium ions adsorption ability of the strain in the cadmium contaminated soils. [Results] The maximum tolerance of NTGB29 to NaCl, pH and concentration of Cd2+ was 10%, 11.0 and 50 mg/L, respectively. The optimum cadmium adsorption conditions were as follows: initial pH 6.4, culture temperature 37 °C, NaCl concentration 4.2%, liquid volume 50 mL in the250 mL flask, and culture time 24 h. Under the conditions, the cadmium ions adsorption percentage reached to79.70%. In addition, the available cadmium ions of the cadmium contaminated soils was decreased significantly by adding strain NTGB29, and the adsorption percentage was 29.65%. [Conclusion] Strain NTGB29 has good environmental resistance and Cd2+ adsorption capacity, and it could be a kind of valuable strain source to develop the heavy metal contaminated soil conditioners and microbial functional agents.

【基金】 国家重点研发计划(2017YFD0200806-4);湖南省重点研发计划(2017NK2144)~~
  • 【文献出处】 微生物学报 ,Acta Microbiologica Sinica , 编辑部邮箱 ,2019年07期
  • 【分类号】X53;X172
  • 【网络出版时间】2018-11-29 13:27
  • 【下载频次】187
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