【摘要】 采用盆栽试验研究了生物质炭配施不同用量化肥氮对烤烟根区土壤微生物群落功能多样性的影响。研究结果显示,添加生物质炭提高了土壤有机碳和微生物生物量碳含量;减少15%化肥氮配施生物质炭处理(T3)显著提高了土壤过氧化氢酶活性,提升了土壤新陈代谢水平。不同处理土壤微生物的碳源利用率不同,以T3处理的AWCD(平均颜色变化率)值最高,且微生物丰富度和优势度均为较高水平。主成分和热图分析表明,不同处理微生物功能差异性和优势碳源不同,其中T3处理的微生物群落功能差异性较小,优势碳源羧酸类中包含的碳源种最多。添加生物质炭可提高烟株根冠比23.06%～42.36%。因此,添加生物质炭可增强植烟土壤微生物活性,提高土壤微生物功能多样性,以减少15%化肥氮配施生物质炭效果最好。更多还原

【Abstract】 A pot experiment was conducted to study the effects of biochar combined with different dosages of nitrogen fertilizer on the functional diversity of soil microbial community in flue-cured tobacco rhizosphere. The results showed that biochar application increased the contents of soil organic carbon and microbial biomass carbon, and the treatment of biochar with 15% reduction of nitrogen fertilizer(T3) significantly improved soil catalase activity and metabolism level. Carbon source utilization of soil microorganism were different under different treatments, T3 treatment had the highest average well color development(AWCD) value, and smaller soil microbial richness and dominance. Principle component analysis and thermal graph analysis showed that the functional differences and dominant carbon sources of soil microbial communities were different under different treatments, among of which, T3 treatment had smaller functional difference and contained most carbon sources in the dominant carbon source carboxylic acids. The biochar application improved root-crown ratio of tobacco by 23.06%–42.36%. Therefore, biocar application can enhance the microbial activity of tobacco-planting soil and improve the diversity of soil microbial function, and the biochar combing with 15% reduction of nitrogen fertilizer has the best effect.更多还原