【摘要】 接种菌根真菌作为重金属污染植物修复生物强化技术之一,已引起研究者的广泛关注。以芦苇（Phragmites australis）-菌根真菌共生系统为研究对象,研究水培条件下,0.02、1、5 mg·L^-1 Cu胁迫条件下接种摩西管柄囊霉（Funneliformis mosseae）及根内球囊霉（Rhizophagus irregularis）对芦苇铜吸收及抗铜能力的影响。结果表明,芦苇茎叶及根系的生长发育均会受到铜胁迫的抑制,然而与茎叶相比,芦苇根系受到铜胁迫的影响更显著。在铜处理浓度为1 mg·L^-1和5 mg·L^-1时,与无菌剂组相比,摩西球囊霉接种组的芦苇根系中的SOD活性分别提高了26.25%、42.3%,而根内球囊霉接种组的SOD活性则呈现下降趋势。接种根内球囊霉使芦苇体内的铜浓度升高,在铜处理浓度为1 mg·L^-1时其茎叶及根系中的铜浓度比无菌剂组分别高出80.03%、33.6%,而在5 mg·L^-1时,则分别高出49.43%、8.53%,增幅较摩西球囊霉接种组显著。本研究证实了菌根真菌可以通过促进芦苇的生长及营养元素的吸收,降低Cu毒害,提高其对Cu的吸收效率,结果可为菌根真菌强化植物修复重金属污染环境提供新的参考。更多还原

【Abstract】 The use of mycorrhizal technology as a bioaugmentation technique for phytoremediation of heavy metal contaminated soil has attracted wide attention of researchers. In this study, the symbiotic system of Phragmites australis mycorrhizal fungi was used as the core to analyze the stress-resistance response process and copper absorption characteristics of symbiotic systems under different copper stress conditions from the aspects of stress resistance and copper absorption efficiency of Phragmites australis. The results showed that the growth and development of stems, leaves and roots of Phragmites australis were inhibited by copper stress.However, compared with stems and leaves, the effects of copper stress on Phragmites australis roots were more significant. When the copper concentration was 1 mg·L^-1 and 5 mg·L^-1, the SOD activity in the roots of Phragmites australis inoculated with Funneliformis mosseae was increased by 26.25% and 42.3%, respectively, compared with the control group. The SOD activity of the Funneliformis mosseae inoculated group showed a downward trend. Inoculation of Rhizophagus irregularis increased the copper concentration in Phragmites australis. When the copper concentration was 1 mg·L^-1, the copper concentration in the stems and leaves of Phragmites australis was higher than the control group by 80.03% and 33.6%, respectively. When the copper concentration was 5 mg·L^-1, they were higher than those of the control group by 49.43% and 8.53%, respectively, and the degree of increase was significantly greater than that of the Funneliformis mosseae inoculated group. This study confirmes that mycorrhizal fungi can promote the growth and nutrient absorption of Phragmites australis, reduce Cu toxicity, increase its absorption efficiency of Cu, and provide a new reference for mycorrhizal fungi to strengthen phytoremediation of heavy metal pollution.更多还原