【摘要】 抗生素抗性基因（AntibioticsResistanceGenes,ARGs）是环境中的一类新型污染物。为了解东江下游地区水源地中ARGs的污染水平及其影响因素,采用实时荧光定量PCR技术,对东江下游地区9个河流型饮用水源地和5个湖泊型饮用水源地8种ARGs[sul1、sul2、sul3、tet（M）、tet（O）、tet（Q）、tet（G）、ermB]的绝对丰度进行检测分析。结果表明:东江下游饮用水源地总ARGs绝对丰度水平偏低,ARGs总丰度范围为2.37×10⁷-4.80×10⁸ copies?L^-1,其中抗性基因sul1的丰度相对较高,这可能与磺胺类药品是常用药品有关。磺胺类ARGs（sul1、sul2）在所有饮用水源地中均有检出,而sul3的检出率为85.7%;四环素类ARGs[tet（M）、tet（O）、tet（Q）、tet（G）]的检出率较高,为64.3%-100%;大环内酯类ARGs检出率最低。河流型水源地上游点位ARGs绝对丰度为1.03×108 copies?L^-1,下游点位绝对丰度为2.89×10⁸ copies?L^-1,因此河流型饮用水源地的ARGs绝对丰度水平随着河流方向呈现逐渐升高趋势。除了一处曾为水产养殖的备用湖泊型水源地外,河流型水源地除sul1外的抗性基因丰度(3.25×10⁵ copies?L^-1)明显高于湖泊型饮用水源地(1.14×10⁵ copies?L^-1)。通过对东江下游典型饮用水源地ARGs进行研究,为该地区ARGs污染现状提供了基础数据,也为水环境中ARGs污染整治提供依据。更多还原

【Abstract】 Antibiotics Resistance Genes（ARGs） is a new type of pollutants in the environment. In order to understand the pollution level of ARGs and its influencing factors in the water sources of the lower Dongjiang River, a real-time fluorescent quantitative PCR technique was used in this study. The absolute abundance of 8 kinds of ARGs [sul1, sul2, sul3, tet（M）, tet（O）, tet（Q）, tet（G）, ermB] in9 river-type drinking water sources and 5 lake-type drinking water sources in the lower reaches of Dongjiang River were detected and analyzed. The results showed that the absolute abundance of total ARGs was low in drinking water sources of the lower Dongjiang River, and the total abundance of ARGs was in the range of 2.37×10⁷-4.80×10⁸ copies?L^-1, in which the abundance of resistant gene sul1 was higher than that of other resistant genes, which may be related to the common use of sulfonamides.Sulfonamides ARGs（sul1, sul2） was detected in all drinking water sources, while sul3 was detected in the rate of 85.7%, the detection rate of tetracycline ARGs [tet（M）, tet（O）, tet（Q）, tet（G）] was high（64.3%-100%） and macrolide ARGs was the lowest. The absolute abundance of ARGs was 1.03×10⁸ copies?L^-1 at upstream and 2.89×10⁸ copies?L^-1 at downstream of river-type water source.Therefore, the absolute abundance level of ARGs in river-type drinking water sources gradually increased with the river’s direction.The average ARGs abundance of river-type drinking water source was 3.25×10⁵ copies?L^-1, and that of lake-type drinking water sources was 1.14×10⁵ copies?L^-1 when sul1 was excluded, besides the highest was 1.41×10⁶ copies?L^-1, which was used for aquaculture before becoming a reserve water source. Therefore, ARGs abundance of river-type water source was higher than that of lake-type drinking water source on the whole. This study of ARGs in a typical drinking water source in the lower Dongjiang River not only provides the basic data for the present study of ARGs pollution in this area, but also support the management and regulation of ARGs pollution in the water environment.更多还原