【摘要】 为研究外生菌根真菌褐环乳牛肝菌(Suillus luteus)对樟子松苗木耐盐性的影响,采用打孔灌根的接种方法对樟子松苗木接种褐环乳牛肝菌,用盆栽试验研究不同含盐量(0.1g·kg-1和0.2g·kg-1NaCl)胁迫10d后褐环乳牛肝菌对樟子松苗木的菌根侵染情况以及樟子松苗木针叶电导率、丙二醛、保护酶活性、可溶性糖、可溶性蛋白、脯氨酸等生理指标的变化,以相同浓度盐胁迫下不接种褐环乳牛肝菌的樟子松苗木为对照。结果表明:试验选用的不同浓度NaCl胁迫,并未影响樟子松外生菌根的合成。接种褐环乳牛肝菌樟子松苗木不同盐浓度处理间苗高、地径、鲜重、干重差异不显著,与不接种樟子松苗木盐胁迫的苗高、鲜重、干重差异显著,地径无显著性差异。接种褐环乳牛肝菌樟子松苗木针叶的相对电导率低于不接种苗木,盐胁迫下接种处理与不接种处理间差异显著;盐胁迫下不接种苗木丙二醛含量高于接种苗木92%以上;盐处理中,接种褐环乳牛肝菌樟子松苗木过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性均高于不接种处理,增幅分别为10.70%～407.69%,84.45%～158%,4.79%～8.57%;盐胁迫增加了苗木可溶性糖、可溶性蛋白含量,可溶性糖增加13.20%～29.53%,可溶性蛋白增加9.64%～13.43%;接种处理降低了苗木脯氨酸含量,降低幅度为18.24%～25.30%。可见,外生菌根菌褐环乳牛肝菌与樟子松形成菌根后提高了樟子松苗木对NaCl的耐受性。更多还原

【Abstract】 To study the effect of Suillus luteus on salt resistance of Pinus sylvestris var. mongolica seedlings, a greenhouse experiment was carried out by drilling and root irrigation with or without Suillus luteus at 2 NaCl levels(0.1 g·kg-1and 0.2 g·kg-1).After 10 d, the mycorrhizal infection status was observed, and the physiological index changes as needles electrical conductivity,malondialdehyde, protective enzyme activities, contents of soluble sugar, soluble protein, and proline were recorded. The result showed that the mycorrhizal synthesis was not affected by different salt stress. And after Suillus luteus inoculation, there were no significant differences between the two NaCl levels on seedlings height, dry and fresh weight and ground diameter. Compared with non-inoculated seedlings, those indexs had significant differences except ground diameter. Under salt stress, needles electrical conductivity was significantly declined after inoculation. The content of malondialdehyde was 92% higher than non-inoculated seedlings. The CAT, POD, SOD activity were 10.7%-407.69%, 84.45%-158%, 4.79%-8.57% higher than non-inoculated seedlings respectively. The content of soluble sugar and soluble protein respectively increased 13.20%-29.53%, 9.64%-13.43% under salt stress. The inoculation decreased proline content by 18.24%-25.30%. The results presented here indicate that the mycorrhiza synthetised from Suillus luteus and the seedlings could improve seedlings salt resistance.更多还原