【摘要】 目的研究新物体识别实验在ICR小鼠学习记忆能力评价中的应用效果。方法将40只ICR小鼠,雌雄各半,随机分为4组,方雌组(方形行为箱雌性小鼠组)、方雄组、圆雌组和圆雄组。按照相同的新物体识别实验流程,测定各组小鼠对物体的探索时间。结果方雌组与方雄组,圆雌组与圆雄组比较,探索时间和对新物体的分辩比均无显著性差异(P>0.05)。方组(方雌组+方雄组)与圆组(圆雌组+圆雄组)比较,对新物体的分辩比无显著性差异(P>0.05),但圆组的总探索时间显著长于方组(P<0.01)。结论进行新物体识别实验时,性别不影响小鼠学习记忆能力的评价,选择圆形行为箱进行实验可更好地评价小鼠学习记忆能力。更多还原

【Abstract】 Object To investigate the effects of the result of novel object recognition tests in different experimental conditions with ICR mice. Method Divided 40 ICR mice(half male and female)into 4 groups randomly, the square female group(the female mice group working in the square box), the square male group, the round female group and the round male group. According to the same experimental process of novel object recognition, the exploration time of mice in each group was measured. Result There was no significant differences between the square female group and the square male group, the round female group and the round male group in the total exploration time and the discrimination ratio of novel object(P>0.05). Compared the square group(the square female group+the square male group) with the round group(the round female group + the round male group), there was no significantly differences in the discrimination ratio of novel object(P>0.05), but the total exploration time of the round group was significantly longer than that of square group(P<0.01). Conclusion In the experiment of novel objects, the sex of mice did not affect the evaluation of learning and memory ability of mice, and the choice of round behavior box for experiments could better evaluate the learning and memory ability of mice.更多还原