【摘要】 近年来,基于高通量测序技术对植物根际土壤微生物菌群结构的测定结果表明,土壤中绝大多数微生物难以通过传统的富营养培养基实现培养和分离。为进一步探索分离土壤中寡营养微生物的方法,结合多种培养因素,以土壤浸出液为基础培养基设计寡营养培养基分离土壤细菌菌株。并通过菌株的细胞形态、生理生化特征、16S rRNA和gyrB基因序列等对其中一株菌进行鉴定,同时对其蛋白酶与铁载体的产生能力进行检测以评价其在植物根际促生方面的应用潜力。利用土壤浸出液做培养基是有效分离土壤寡营养细菌的方法 ;编号为S35的菌株鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia),具有蛋白酶和铁载体产生能力。采用的寡营养培养基可以分离获得在富营养培养基上不易分离的细菌,得到S. maltophilia S35菌株在植物促生方面具有一定的应用潜力。更多还原

【Abstract】 In recent years,based on high-throughput sequencing technology,the determination of microbial flora structure in plant rhizosphere soil shows that most microorganisms in soil are difficult to culture and separate via traditional eutrophic medium. It is aimed to further explore an effective method for isolating oligotrophic microorganisms from soil. A variety of factors were combined to design an oligotrophic medium to isolate soil bacteria based on soil leaching solution. One of the strains was identified by cell morphology,physiological and biochemical characteristics,16 S rRNA,and gyrB gene sequences;meanwhile,its function of proteases-producing and siderophoreproducing was detected to assess the potential in promoting plant growth. Using soil leaching solution as a medium was a method for effectively isolating soil oligotrophic bacteria,and the strain numbered S35 was identified as Stenotrophomonas maltophilia,it had the ability to produce protease and siderophore. The oligotrophic medium used in this study can be used to obtain bacteria that are difficult to isolate through nutrientrich medium. In addition,S. maltophilia S35 has certain application potential in plant growth promotion.更多还原