【摘要】 目的:探讨长链非编码RNA SNHG3对人乳腺癌细胞MCF-7增殖、迁移与侵袭的影响。方法:构建SNHG3过表达质粒,实验分别设置阴性对照组(pcDNA-3. 1+)与SNHG3基因过表达组(pcDNA-3. 1+/SNHG3)。将MCF-7细胞转染对照组质粒和SNHG3过表达质粒,采用实时定量PCR方法检测SNHG3 mRNA转录水平,Western blot检测MMP9及EMT相关蛋白质水平;集落形成实验检测MCF-7细胞增殖能力;划痕愈合实验检测MCF-7细胞横向迁移能力; Transwell小室实验检测MCF-7细胞纵向迁移能力及侵袭能力。结果:过表达SNHG3后,MCF-7细胞中SNHG3的mRNA水平显著增高(P <0. 001); MCF-7细胞的体外增殖能力明显增加(P <0. 01),迁移(P <0. 01)与侵袭能力(P <0. 001)也显著增强,实时定量PCR,Western blot结果显示SNHG3可激活EMT相关通路。结论:过表达SNHG3可能通过激活EMT通路促进乳腺癌MCF-7细胞的增殖,迁移与侵袭。更多还原

【Abstract】 Objective: To investigate the effects of LncRNA SNHG3 on the proliferation,migration and invasion of human breast cancer MCF-7 cells. Methods: The recombiant plasmid of SNHG3 was constucted. The experiment was divided into two group: control group( transfected with pcDNA-3. 1 + plasmid) and treatment group( transfected with pcDNA-3. 1 +/SNHG3 plasmid). The mRNA level of SNHG3 was detected by qRTPCR; The mRNA and protein levels of MMP9,EMT-related makers were measured by qRT-PCR and Western blot; The colony formation assay was used to test the proliferation of MCF-7 cells; The wound-healing and Transwell chamber assay were taken to evaluate the migration and invasion of MCF-7 cells. Results: The expression of SNHG3 in MCF-7 cells was highly upregulated compared with control group( P < 0. 001);Furthermore,The mRNA and protein levels of MMP9 and EMT-related markers were increased; The migration and invasion abilities of SNHG3 overexpression group measured by wound healing assay and transwell chamber assay were augmented significantly. And the colony formation assay showed that proliferation of treatment group were increased remarkably. Conclusion: Overexpression of SNHG3 may promote the proliferation,migration and invasion of breast cancer MCF-7 cells by activating the EMT signaling pathway.更多还原