【摘要】 乌鲁木齐10号泉起源于天山山脉博格达峰,地下径流于地震断裂带,泉水中硫化氢、甲烷等地球化学元素迁移活跃,为揭示水文地球化学变化对地震断裂带泉水细菌群落结构的影响,本研究对该泉水样品定期采样,以微孔滤膜收集菌体,使用SDS-酶解法提取总DNA,对细菌16S rDNA V3区进行PCR-DGGE分析并对条带测序,条带的光密度信息与监测的地球化学指标进行典型相关分析(CCA)。结果表明B16(Uncultured bacterium)和ε-变性菌纲(epsilon proteobacterium)与氟含量成正相关;黄杆菌B1(Flavobacterium)、绿脓假单胞菌(Pseudomonas aeruginosa)、B10(Uncultured bacterium)和腐生性葡萄球菌(Staphylococcus saprophyticus)与硫化物呈正相关;硫微螺菌(Thiomicrospira arctica)、黄杆菌B3(Flavobacterium)及阿尔莱葡萄球菌(Staphylococ-cus arlettae)与氢气呈正相关。断裂带泉水细菌能够对地层深处渗透出的地球化学元素产生灵敏的应答。更多还原

【Abstract】 Seismic fault belt is a main passageway of various kinds of gas and geochemistry elements,where are important zone for matters,energy and information transfer between lithosphere and atmosphere.Microbes living in the belt effected intensively by the hydrogeochemical changes caused by earth crust movement.The 10th spring of Urumq originates from Bogeda mountain of Tianshan mountain range,and the groundwater runoff in seismic fault belt.The spring water contains various geochemistry elements,such as sulfide,methane,hydrogen,radon,sulfide,methane,carbon dioxide,helium,fluorine and hydrarg et al,many of which may potentially act as electron donors capable of supporting chemolithotrophy-based primary production.In order to reveal the impact of hydrogeochemical changes to bacterial community in seismic fault belt spring water,thirty-one samples of spring water collected from October 12th to November 11th and their corresponding geochemial parameters were determined.Of them,ten samples were used for bacterial dynamic changing monitoring.The planktonic communities were collected by microporous membrane filtration and then total DNA were extracted by SDS-enzymatic disruption method.The V3 region of the 16S rDNA gene of those samples was analyzed by PCR-DGGE(Denaturing Gradient Gel Electrophoresis) and different bands were excised for sequencing.The DGGE fingerprints combined with hydrogeochamical parameters were analyzed by CCA(Canonical correspondence analysis).The results showed that B16(Uncultured bacterium) and epsilon proteobacterium were positive correlate to Fluorine fluctuation and clustered as group one and two,respectively;B1(Flavobacterium),Pseudomonas aeruginosa,B10(Uncultured bacterium) and Staphylococcus saprophyticus were positive correlate to sulfide change;Thiomicrospira arctica,B3(Flavobacterium) and Staphylococcus arlettae were positive correlated to hydrogen content.The result indicated that bacterial communities in seismic fault belt spring water could response sensitively to hydrogeochemical parameter changes,and this may provide a novel means for crust movement monitoring and earthquake forecast.更多还原