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不同浓度NaCl处理对黑果枸杞叶片性状的影响

Effects of NaCl treatment on leaf traits of Lycium ruthenicum

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【作者】 杨万鹏马瑞杨永义倪强卢瑜马彦军

【Author】 YANG Wanpeng;MA Rui;YANG Yongyi;NI Qiang;LU Yu;MA Yanjun;Forestry College, Gansu Agriculture University;

【通讯作者】 马彦军;

【机构】 甘肃农业大学林学院

【摘要】 通过黑果枸杞叶片性状对盐处理的响应来探讨其耐盐特征。以格尔木1年生黑果枸杞苗为实验材料,采用不同浓度NaCl处理(50、100、150、200、250 mmol·L-1),并设置短期(15 d)与长期(30 d)两个时间段,测定并分析黑果枸杞叶片性状不同指标对盐处理的响应。结果表明,当黑果枸杞在NaCl处理30d时,叶片含水量、叶片体积、叶片数和叶片生物量均表现出先增大后减小的趋势,且与对照差异显著,而处理15d则变化不明显。当黑果枸杞受到NaCl处理时,随着NaCl浓度的增大,鲜叶密度和干叶密度则表现出先增后减再增的趋势。同时,叶片含水量、叶片体积、叶片数和叶片生物量各指标均表现出在NaCl为中浓度(150mmol·L-1)时,对应各指标值均达到最大值,相反低浓度(50mmol·L-1)和高浓度(250 mmol·L-1)下相比对照均有所下降。在生物量与叶片各指标回归分析中可知,叶片含水量、叶片体积和叶片数与叶片生物量之间显著相关,其余各指标与叶片生物量间相关性不显著。通过主成分分析筛选出叶片体积、干叶密度、鲜叶密度和叶片含水量作为评价盐胁迫对黑果枸杞叶片影响的参考指标,为黑果枸杞耐盐新品种选育提供形态学方面的依据。

【Abstract】 In this paper, the salt tolerance characteristics of Lycium ruthenicum were studied by the response of leaf characters to salt stress. One-year-old Golmud Lycium ruthenicum seedlings were treated with different concentrations of NaCl(50,100,150,200,250 mmol·L-1), and the responses of leaf traits of Golmud Lycium ruthenicum to salt treatment were measured and analyzed in two periods of short-term(15 days) and long-term(30 days). The results showed that the leaf water content, leaf volume, leaf number and leaf biomass of Lycium ruthenicum increased first and then decreased after 30 days of NaCl treatment, and the difference was significant compared with the control, but the change was not obvious after 15 days of NaCl treatment. When Lycium ruthenicum was treated with NaCl, the density of fresh leaves and dry leaves increased first, then decreased and then increased with the increase of NaCl concentration. Meanwhile, the leaf water content, leaf volume, leaf number and leaf biomass were all maximized at NaCl medium concentration(150 mmol·L-1), whereas those at low concentration(50 mmol·L-1) and high concentration(250 mmol·L-1) decreased compared with the control. Regression analysis of leaf biomass and leaf indexes showed that leaf water content, leaf volume and leaf number were significantly correlated with leaf biomass, while the correlation between other indexes and leaf biomass was not significant. Leaf volume, dry leaf density, fresh leaf density and leaf water content were selected by principal component analysis as the reference indexes to evaluate the effects of salt stress on leaves of Lycium ruthenicum and it will provide morphological basis for breeding new varieties of Lycium ruthenicum.

【基金】 国家自然科学基金(31560215,31760244);中国博士后科学基金(2017M613295XB);甘肃农业大学青年研究生指导教师扶持基金项目(GAU-QNDS-201712);甘肃省GEF/OP12三期项目专题研究项目:黑果枸杞吸盐特征及对盐碱地土壤改良效果研究(GS-GEF/OP12-02)
  • 【文献出处】 生态科学 ,Ecological Science , 编辑部邮箱 ,2019年04期
  • 【分类号】S567.19
  • 【下载频次】140
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