【摘要】 将体质量(110±5 g)的大口黑鲈幼鱼饲养在室内100 L白色PVC水桶中,每个桶6尾,在水温21, 25, 29℃和33℃的环境下驯养2周,测定肌肉、肝脏、鳃和肠道中抗氧化酶活性以及丙二醛(MDA)、谷胱甘肽(GSH)、热休克70蛋白(HSP70)和金属硫蛋白(MT)含量,以研究温度对大口黑鲈幼鱼不同组织抗氧化能力及免疫相关蛋白表达的影响。结果显示,在21—33℃条件下,实验鱼超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均在鳃中最高且变化较大,整体随温度升高而先降低后升高,在29℃组最低。MDA含量在鳃中较高,肠中次之,均在21℃组含量最高。肝脏,鳃和肠中均含有较高GSH,整体随温度升高而呈先降低后升高的趋势。肝脏中总抗氧化能力(T-AOC)处于较高,肠中次之,肌肉中最低,整体随温度升高呈波动性变化,在29℃组最低。HSP70含量在肌肉、肝脏和肠中较高,整体随着温度升高而先升高后降低。MT含量在肌肉和肝脏中较高,不同温度组中MT含量变化不大。结果表明,长期温度胁迫对大口黑鲈幼鱼产生一定应激损伤,引起其机体免疫水平降低,并且高温对其应激损伤大于低温;大口黑鲈幼鱼肝脏、鳃和肠道组织对温度胁迫更敏感。更多还原

【Abstract】 Juvenile Micropterus salmoides with initial body weight of(110±5 g) g was randomly allocated into 4 temperature groups(21, 25,29℃ and 33℃)in 2 weeks to investigate the effects of temperature on antioxidant capacity and immune associated protein expression in different tissues and organs(white muscle, liver, gill and intestine) of juvenile M. salmoides. Parameters analyzed included superoxide dismutase(SOD), catalase(CAT) activity, malondialdehyde(MDA), glutathione(GSH), total antioxidant capacity(T-AOC), heat shock protein70(HSP70) and metallothionein(MT) content. Results showed that as the temperature ranged from 21℃ to 33℃, the SOD and CAT activity presented earlier decreased and later increased trend, and obtained the minimum value at 29℃. The content of MDA was highest at 21℃ and accumulated in gill and intestine. GSH expressed in various tissues of juvenile M. salmoides, including liver, gill and intestine and presented upward parabola trend. T-AOC also obtained the minimum value at 29℃ and presented a decreasing tendency from liver to intestine and to muscle. HSP 70 was higher in liver, gill and intestine, and revealed in the tendency of parabola. MT was higher in muscle and liver, and showed no great change between different temperature groups. In conclusion, temperature stress does have some effects on liver,gill and intestine of juvenile M. salmoides, leading decreased immunity. High temperature causes more severe stress damage than low temperature. Liver, gill and intestine seem to be more sensitive to temperature stress than muscle.更多还原