【摘要】 碳纳米管的生态安全和健康风险日益受到人们的广泛关注。本文采用典型的海洋底栖生物——太平洋牡蛎（Crassostrea gigas, C. gigas）作为受试生物,研究了单壁碳纳米管（Single-Walled Carbon Nanotubes, SWCNTs）暴露对其造成的毒性效应及牡蛎自身的防御机制,以期为碳纳米管的海洋生态风险评价提供科学依据。在0.1～10 mg·L^-1的SWCNTs暴露96 h后,太平洋牡蛎鳃和消化腺中的丙二醛（malondialdehyde, MDA）含量显著增加（P≤0.05）,总超氧化物歧化酶（superoxide dismutase, SOD）和过氧化氢酶（catalase, CAT）活性呈现显著的剂量依赖性升高（P≤0.05）,cat、hsp70、aox及caspase-7等基因的相对表达量显著上调（P≤0.05）。相比于单独暴露,P-gp蛋白抑制剂Tariquidar与SWCNTs的复合暴露显著增加了鳃和消化腺中MDA含量,产生了更严重的氧化损伤。这些结果表明,SWCNTs暴露对太平洋牡蛎的鳃和消化腺造成了一定程度的氧化损伤,而牡蛎体内的抗氧化系统和多外源性物质抗性机制在防御SWCNTs的过程中起到了至关重要的作用。更多还原

【Abstract】 The ecological and health risks of carbon nanotubes are receiving more attention. In this study, Pacific oyster(Crassostrea gigas), a kind of typical marine benthic organism, was exposed to single-walled carbon nanotubes(SWCNTs) to investigate the toxicity induced by SWCNTs and the defense mechanism of C.gigas. After 96-h exposure to 0.1-10 mg L-1 SWCNTs, the content of malondialdehyde(MDA) in gills and digestive glands increased significantly(P≤0.05), the activities of total superoxide dismutase(SOD) and catalase(CAT) showed the significant dose-dependent increase(P≤0.05), and the relative expressions of genes, i.e., cat, hsp70, aox and caspase-7, were significantly up-regulated(P≤0.05). The co-exposure of Tariquidar(P-gp protein inhibitor) and SWCNTs induced the significant increase of the MDA content in gills and digestive glands(P≤0.05), indicating that more serious oxidative damage were caused by the mixture. The results revealed that the antioxidant system and Multi-xenobiotic Resistance Mechanism played vital roles in the defense system of Pacific oyster against the SWCNTs exposure.更多还原