【摘要】 建立了分子印迹固相萃取-高效液相色谱法测定果汁中展青霉素的方法。浓缩果汁样品经酶解,乙酸-水溶液稀释,分子印迹固相萃取小柱净化后(澄清果汁样品经乙酸-水溶液稀释后,直接进行SPE净化),以Waters XSELECT HSS T3色谱柱为分离柱,以水和乙腈作为流动相,二极管阵列检测器高效液相色谱进行检测,外标法定量。展青霉素在20～1000μg/L质量浓度范围内线性关系良好,相关系数为0.9994,方法的定量限为10.0μg/kg;添加水平为10.0、20.0μg/kg和40.0μg/kg时,加标回收率为83.0%～91.5%,相对标准偏差为2.8%～7.6%。结果表明:该方法准确、高效、特异性好、重现性高,适用于果汁中展青霉素的测定。更多还原

【Abstract】 A method was developed for the determination of patulin in juice by molecularly imprinted solid phase extraction and high performance liquid chromatography. The cloudy juice was hydrolyzed by enzyme and diluted with acetic acid-water solution. The extract of cloudy juice was then enriched and purified by a molecularly imprinted solid-phase extraction(SPE) cartridge. If the juice was clear, it was diluted with acetic acid-water solution and then directly treated by the cartridge as above. The separation was perfoemed on a Waters XSELECT HSS T3 column with the mobile phase of acetonitrile and water.The patulin was detected by high performance liquid chromatography with diode array detector. External standard was used for the quantitative determination of patulin in juice. The good linearity was obtained in the range of 20～1000 μg/L with the correlation coefficient of 0.9994 and the limit of quantitation of 10.0 μg/kg. The average recoveries ranged from 83.0% to 91.5% at three spiked levels of 10.0, 20.0, 40.0 μg/kg with the relative standard deviations of 2.8%～7.6%. It was proved to be a accurate, efficient, specific and reproducible method which is suitable for the determination of patulin in juice.更多还原