【摘要】 将餐厨垃圾中的微生物采用营养琼脂培养基(LA)在温度55℃,相对湿度50%RH的条件下培养,根据菌落形态进行筛选分离。经过筛选得到细菌20株,测定其对淀粉、蛋白质、油脂和纤维素的分解效果,并根据分解效果选出最优菌株X13。对其进行形态学、生理生化以及分子生物学的鉴定并测定生长曲线,最终判定菌株X13为地衣芽孢杆菌(Bacillus licheniformis)。再使用X13菌株制成菌制剂处理餐厨垃圾,在小型餐厨垃圾处理器中运行48h后,测定餐厨垃圾降解率达到72.82%。X13菌株制成的菌制剂能够有效提高餐厨垃圾的降解效率,缩短处理时间,所以该地衣芽孢杆菌可以用于餐厨垃圾处理工业。更多还原

【Abstract】 The efficient degrading and high-temperature resistance bacteria in kitchen waste were isolated according to the characteristics of domestic kitchen waste.The microorganisms in the kitchen waste were cultured for 48 hours under the conditions of a temperature of 55℃ and a relative humidity of 50% RH using a nutrient agar medium(LA),and then they were screened and separated according to the colony morphology.20 strains of bacteria were obtained,and their decomposition effects of starch,protein,oil and cellulose were determined.X13,which has the best decomposition effect,was selected and identified to be Bacillus licheniformis by morphology,physiology,biochemistry and molecular biology.The microbial agent prepared by X13 strain was used to decompose the kitchen waste.After running for 48 hours in the small kitchen waste disposer,the decomposition of the kitchen waste reached 72.82%.The results showed that the microbial agent could effectively improve the decomposition efficiency of the kitchen waste and shorten the decomposition time.Therefore,the B.licheniformis could be used in the kitchen waste decomposition industry.更多还原