【摘要】 以蛋白水解度为指标,利用中性蛋白酶和木瓜蛋白酶酶解蚕蛹蛋白。通过单因素试验和正交试验对影响蚕蛹蛋白水解度的因素进行优化试验,分析了两种蛋白酶对蚕蛹蛋白酶解效果的影响,并测定水解产物抗氧化活性。结果表明,中性蛋白酶酶解蚕蛹蛋白时,最优工艺条件为底物浓度为1.2%、酶解时间3.5 h、酶解pH 10.0、酶与底物比5.0%,此时,蚕蛹蛋白水解度最高为27.38%;木瓜蛋白酶酶解蚕蛹蛋白时,最优工艺条件为底物浓度为1.5%、酶解时间5.0 h、酶解pH 10.0、酶与底物比9.0%,此时,蚕蛹蛋白水解度最高为18.26%。最佳工艺条件下,中性蛋白酶和木瓜蛋白酶酶解产物DPPH清除率分别为83.84%和81.24%,超氧阴离子自由基清除率分别为6.36%和9.72%,羟基自由基清除率分别为43.30%和62.24%。综上所述,木瓜蛋白酶对酶解产物的抗氧化效果较好。更多还原

【Abstract】 Using hydrolysis degree of silkworm pupa protein as the research indicators, neutral proteinase and papain were used for enzymatic hydrolysis of silkworm pupa protein. The single factor test and orthogonal test were used to optimize the factors affecting the degree of hydrolysis of silkworm pupa proteins. The effect of two proteases on the proteolysis of silkworm pupa was analyzed, and the antioxidant activity of the hydrolysate was determined. The results showed the optimum conditions for neutral protease enzyme hydrolysis silkworm pupa protein were as follows, substrate concentration 1.2%,enzymolysis time 3.5 h, enzymolysis pH 10.0, and ratio of enzyme to substrate 5.0%. At this time, the highest degree of hydrolysis of silkworm pupa protein was 27.38%. When papain was used for enzymatic hydrolysis of silkworm pupa protein,the optimum conditions were substrate concentration 1.5%, hydrolysis time 5.0 h, enzymolysis pH 10.0, and ratio of enzyme to substrate 9.0%. The highest degree of hydrolysis of silkworm pupa protein was 18.26%. Under the optimal conditions,the DPPH clearance rates of neutral protease and papain hydrolyzed products were 83.84% and 81.24%, respectively. The scavenging rates of superoxide anion radicals were 6.36% and 9.72%, respectively. And the hydroxyl radical scavenging rates were 43.30% and 62.24%, respectively. In summary, the anti-oxidation effect of papain on the enzymolysis product was better.更多还原