【摘要】 目的建立近交系中国仓鼠遗传质量标准。方法在查阅大量国内外相关文献后,按照实验动物哺乳类实验动物遗传质量控制标准(GB14923-2010),根据群体遗传学的相关理论建立近交系中国仓鼠遗传质量标准。构建中国仓鼠基因组富集文库的方法获得微卫星位点并开发相应的微卫星引物,应用微卫星遗传标记进行遗传质量检测。结果初步建立了中国仓鼠近交系遗传检测方法和质量标准。结论近交系中国仓鼠遗传质量标准的建立,可规范近交系中国仓鼠的饲养与繁殖,推动我国实验动物遗传质量标准体系的进一步完善。更多还原

【Abstract】 Objective To establish genetic quality standard of inbred Chinese hamster(Cricetulus griseus). Methods Based on investigating a large number of related literatures, according to Laboratory animal-Genetic quality control of mammalian laboratory animals(GB14923-2010) and the theory of population genetics, the genetic quality standard of inbred Chinese hamster was established. The genetic quality of Chinese hamster was detected by microsatellite markers which were obtained from a Chinese hamster genomic enrichment library and the PCR primers of them were designed. Results The genetic quality standard and the detection method of inbred Chinese hamster were preliminarily established. Conclusion The establishment of genetic quality standard of inbred Chinese hamster will standardize the breeding and reproduction of inbred Chinese hamster and promote the further improvement of genetic quality standard system of laboratory animals in China.更多还原