【摘要】 探究大孔吸附树脂富集纯化番泻豆荚中番泻苷的最佳工艺。以番泻豆荚为原料,采用高效液相测定番泻苷A、B含量,以吸附率及解吸率为指标,采用静态吸附试验对5种大孔树脂进行筛选,优选出吸附解吸性能最佳的大孔树脂,并对纯化条件进行优化,确定最佳工艺参数。结果表明:AB-8型树脂对番泻豆荚中番泻苷有较好的吸附及解吸附效果,其最佳工艺为:树脂饱和吸附量按生药计为0.21 g/g,径高比为1∶8,上样流速为1 BV/h,洗脱流速为2 BV/h,以3 BV水除杂,3 BV 30%乙醇洗脱,纯化后产品中番泻苷A、B总含量高达26.05%。结论:AB-8型树脂适合富集纯化番泻豆荚中番泻苷。更多还原

【Abstract】 To explore the optimal technology for enrichment and purification of sennoside from senna pod with macroporous resin. Methods: The content of sennaside A and B were determined by the HPLC. By using adsorption rate and desorption rate as the indices, five kinds of macroporous resins were screened by the static adsorption test. The best macroporous resin with better adsorption and desorption properties, and the optimal purification conditions of resin were obtained based on static adsorption. Results: Experiment results showed that AB-8 resin possessed high absorption capacity. The best technical parameters were obtained as follows: saturation adsorption capacity of resins 0.21 g/g(equivalent as crude drug), the ratio of diameter to height 1∶8, absorption flow rate 1 BV/h, desorption flow rate 2 BV/h, 3 BV water impurity, eluent with 3 BV 30% ethanol. The total content of sennoside A and B in the purified product were as high as 26.04%. Conclusion: The AB-8 macroporous adsorption resin has the best enrichment and purification efficiency for sennoside from senna pod.更多还原