【摘要】 采用Illumina Miseq高通量测序平台,对木霉LTR-2拌种剂拌种小麦后不同发育时期小麦根际土壤真菌进行测序,分析群落丰富度、多样性以及结构。结果表明,木霉拌种处理小麦可以增加小麦根际真菌群落的丰富度,对真菌群落的均匀度影响不明显,但真菌群落的优势度有明显的增加;木霉拌种处理后小麦根际土壤真菌的菌群结构构成相近,没有发生明显变化,但各真菌类群所占比例发生了明显的变化,其中一些病原真菌如链格孢属、赤霉属、镰刀菌属所占比例较不拌种处理均有所降低。该研究为木霉菌LTR-2防治小麦土传病害的生防机制提供了理论依据。更多还原

【Abstract】 The species richness,diversity and composition of fungal community in wheat rhizosphere soil at different developmental stages with Trichoderma LTR-2 seed dressing agent were analyzed using Illumina MiSeq high-throughput sequencing platform. The results demonstrated that Trichoderma treatment of wheat seed increased the species richness of fungal community in the rhizosphere,and the community dominance increased significantly,while no obvious changes in the community uniformity were observed. In addition,the community composition was found similar with and without Trichoderma treatment. However,the proportion of various fungal groups in the rhizosphere changed significantly with Trichoderma treatment,among which Alternaria spp.,Gibberella spp.,and Fusarium spp. were decreased compared to non-seed dressing treatment. All the results provide a theoretical basis for better understanding the biocontrol mechanism of Trichoderma sp. LTR-2 against wheat soil-borne diseases.更多还原